Alterations in Gastric Microbiota After H. Pylori Eradication and in Different Histological Stages of Gastric Carcinogenesis.
Alterations in Gastric Microbiota After H. Pylori Eradication and in Different Histological Stages of Gastric Carcinogenesis.
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DOI：
10.1038/srep44935
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发表时间：
2017-03-21
影响因子：
4.6
通讯作者：
Leung WK
中科院分区：
文献类型：
--
作者：
Li TH;Qin Y;Sham PC;Lau KS;Chu KM;Leung WK
幽门螺杆菌（HP）以外的细菌在胃中的作用仍然难以捉摸。我们对胃癌发生不同组织学阶段和接受HP根除治疗后的个体的胃微生物群进行了表征。从HP胃炎、胃肠上皮化生（IM）、胃癌（GC）受试者和HP阴性对照受试者中获得内镜胃活检。通过靶向16 S rDNA的Illumina MiSeq平台表征胃微生物群。除优势H. pylori中，我们观察到其他变形菌门，包括嗜血杆菌属、沙雷氏菌属、奈瑟氏菌属和寡养单胞菌属作为人胃微生物群的主要组分。虽然样品根据HP的相对丰度在很大程度上收敛，但回收了GC和其他样品的清晰分离。虽然HP相对丰度和细菌多样性之间存在很强的负相关性，但这种相关性在GC样品中较弱，与具有类似HP水平的其他样品相比，GC样品往往具有较低的细菌多样性。根除HP导致细菌多样性增加，其他细菌的相对丰度恢复到与没有HP的个体相似的水平。总之，HP定植导致胃微生物群的改变和细菌多样性的减少，这可以通过抗生素治疗来恢复。
The role of bacteria other than Helicobacter pylori (HP) in the stomach remains elusive. We characterized the gastric microbiota in individuals with different histological stages of gastric carcinogenesis and after receiving HP eradication therapy. Endoscopic gastric biopsies were obtained from subjects with HP gastritis, gastric intestinal metaplasia (IM), gastric cancer (GC) and HP negative controls. Gastric microbiota was characterized by Illumina MiSeq platform targeting the 16 S rDNA. Apart from dominant H. pylori, we observed other Proteobacteria including Haemophilus, Serratia, Neisseria and Stenotrophomonas as the major components of the human gastric microbiota. Although samples were largely converged according to the relative abundance of HP, a clear separation of GC and other samples was recovered. Whilst there was a strong inverse association between HP relative abundance and bacterial diversity, this association was weak in GC samples which tended to have lower bacterial diversity compared with other samples with similar HP levels. Eradication of HP resulted in an increase in bacterial diversity and restoration of the relative abundance of other bacteria to levels similar to individuals without HP. In conclusion, HP colonization results in alterations of gastric microbiota and reduction in bacterial diversity, which could be restored by antibiotic treatment.