Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine.
Differentiation of human promyelocytic leukemia cells in vitro by 6-thioguanine.
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6-硫鸟嘌呤对人早幼粒细胞白血病细胞的体外分化。
DOI：
10.1016/0304-3835(80)90062-2
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发表时间：
1980
期刊：
影响因子：
9.7
通讯作者：
Sartorelli,AC
中科院分区：
文献类型：
--
作者：
Papac,RJ;Brown,AE;Schwartz,EL;Sartorelli,AC
抗肿瘤剂 6-硫鸟嘌呤被发现是体外 HL60 人白血病细胞分化的高效诱导剂，根据形态、功能和细胞化学标准判断，产生的细胞主要具有骨髓特征。 HL60 的成熟是可以实现的，并且根据细胞数量测量没有显着的细胞毒性；此外，最大分化发生在嘌呤抗代谢物水平低于产生50%生长抑制的水平。 6-硫鸟嘌呤诱导的分化细胞具有独特的粒细胞形态，并且能够在成熟吞噬细胞典型的呼吸爆发期间产生超氧阴离子。
The antineoplastic agent, 6-thiogaunine, was found to be a highly potent inducer of differentiation of HL60 human leukemia cells in vitro, producing cells with predominantly myeloid characteristics, as judged by morphological, functional, and cytochemical criteria. Maturation of HL60 was attainable without significant cytotoxicity as measured by cell number; moreover, maximal differentiation occurred at a level of the purine antimetabolite below that producing 50% growth inhibition. The 6-thioguanine-induced differentiated cells had distinctive granulocytic morphology, and were capable of generating superoxide anion during the respiratory burst typical of mature phagocytic cells.