Millisecond-timescale, genetically targeted optical control of neural activity
Millisecond-timescale, genetically targeted optical control of neural activity
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DOI：
10.1038/nn1525
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发表时间：
2005-09-01
影响因子：
25
通讯作者：
Deisseroth, K
中科院分区：
文献类型：
--
作者：
Boyden, ES;Zhang, F;Deisseroth, K
定义明确的神经元种群中对活动的时间精确，无创控制是系统神经科学的长期目标。我们为此目的适应了天然存在的藻类蛋白通道蛋白-2（一种快速门控的光敏阳离子通道），通过使用慢病毒基因递送与高速光学切换到光稳定的哺乳动物神经元结合使用。我们证明了对神经元尖峰的可靠，毫秒的控制，以及对兴奋性和抑制性突触传播的控制。这项技术允许使用光来改变单个尖峰和突触事件水平的神经处理，从而为神经科学家和生物医学工程师提供广泛适用的工具。
Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers.