Molecular analysis of neurogenic placode development in a basal ray-finned fish.

Molecular analysis of neurogenic placode development in a basal ray-finned fish.
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基础射线鳍鱼神经源性基板发育的分子分析。

DOI:
10.1002/dvg.20707
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发表时间:
2011-04
期刊:
影响因子:
1.5
通讯作者:
Baker, Clare V. H.
Baker, Clare V. H.
中科院分区:
生物学4区
文献类型:
--
作者:
Modrell, Melinda S.;Buckley, David;Baker, Clare V. H.

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神经源性基板是胚胎脊椎动物头部外胚层的短暂增厚的斑块,产生成对的外周感觉器官和颅感觉神经节中的大多数神经元。我们提出了第一个分析神经原基板的发展过程中的基因表达在基底radiopterygian(射线鳍鱼),北美白鲟(Polyodon spathula)。Pax 3在深基板中的表达证实了其与眼三叉神经基板的同源性。我们报告的保护Pax 2和Pax 8的表达在耳和/或上鳃基板,Phox 2b的上鳃基板衍生的神经元,Sox 3在上鳃和侧线基板的发展,和NeuroD在发展中的颅感觉神经节。我们确定Sox 3作为一种新的标记,发展领域的电感壶腹器官和壶腹器官本身。Sox 3也是第一个用于区分鳍状软骨壶腹器官的分子标记。这是一致的,但不能证明,侧线基板起源为actinopterygian壶腹器官。
Neurogenic placodes are transient, thickened patches of embryonic vertebrate head ectoderm that give rise to the paired peripheral sense organs and most neurons in cranial sensory ganglia. We present the first analysis of gene expression during neurogenic placode development in a basal actinopterygian (ray-finned fish), the North American paddlefish (Polyodon spathula). Pax3 expression in the profundal placode confirms its homology with the ophthalmic trigeminal placode of amniotes. We report the conservation of expression of Pax2 and Pax8 in the otic and/or epibranchial placodes, Phox2b in epibranchial placode-derived neurons, Sox3 during epibranchial and lateral line placode development, and NeuroD in developing cranial sensory ganglia. We identify Sox3 as a novel marker for developing fields of electrosensory ampullary organs and for ampullary organs themselves. Sox3 is also the first molecular marker for actinopterygian ampullary organs. This is consistent with, though does not prove, a lateral line placode origin for actinopterygian ampullary organs.
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