Near-IR-Absorbing Gold Nanoframes with Enhanced Physiological Stability and Improved Biocompatibility for In Vivo Biomedical Applications.
Near-IR-Absorbing Gold Nanoframes with Enhanced Physiological Stability and Improved Biocompatibility for In Vivo Biomedical Applications.
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DOI：
10.1021/acsami.6b12591
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发表时间：
2017-01
影响因子：
9.5
通讯作者：
Liying Wang;Yunching Chen;Hsin-Yao Lin;Y. Hou;Ling‐Chu Yang;Aileen Y. Sun;Jia-yu Liu;Chien-Wen Chang;D. Wan
中科院分区：
文献类型：
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作者：
Liying Wang;Yunching Chen;Hsin-Yao Lin;Y. Hou;Ling‐Chu Yang;Aileen Y. Sun;Jia-yu Liu;Chien-Wen Chang;D. Wan
本文描述了近红外（NIR）吸收金纳米框架（GNF）的合成，并系统研究了其与中空金银纳米壳（GNS）的生理稳定性和生物相容性，后者由于其在近红外区域的局域表面等离子体共振（LSPR）而被广泛用作生物医学应用中的光热剂。使用银纳米球（SNP）作为起始材料，分三个步骤合成 GNF：电置换、Au 沉积和 Ag 脱合金。 GNF 的形貌和光学性质取决于 Au 涂层的厚度和 Ag 脱合金的程度。最佳的 GNF 表现出由几个厚边缘组成的坚固的球形骨架，但在近红外区域保留了独特的 LSPR 吸光度，即使骨架内的 Ag 含量仅为 10 wt%，比 GNS 低 4 倍。这些GNF表现出诱人的光热转换能力和良好的光热稳定性，可以通过光诱导加热有效杀死4T1癌细胞。此外，GNF 在生物介质（例如盐水、血清）中孵育后保留了其形态和光学特性，而 GNS 在相同条件下不稳定，因为大量的银含量随壳快速溶解。此外，GNF 与正常细胞（例如 NIH-3T3 和肝细胞；两种细胞的细胞活力：>90%）具有良好的生物相容性，而 GNS 表现出显着的剂量依赖性细胞毒性（例如，1.14 nM 时肝细胞的细胞活力：约 11%），并伴有反应性诱导 氧物种。最后，GNFs在体内小鼠模型中表现出良好的生物相容性和生物安全性；相反，GNS 的积累会导致肝损伤和炎症。我们的结果表明，GNF 具有作为稳定、生物相容性近红外光吸收剂用于体内应用（包括癌症检测和联合治疗）的巨大潜力。
This paper describes the synthesis of near-infrared (NIR)-absorbing gold nanoframes (GNFs) and a systematic study comparing their physiological stability and biocompatibility with those of hollow Au-Ag nanoshells (GNSs), which have been used widely as photothermal agents in biomedical applications because of their localized surface plasmon resonance (LSPR) in the NIR region. The GNFs were synthesized in three steps: galvanic replacement, Au deposition, and Ag dealloying, using silver nanospheres (SNP) as the starting material. The morphology and optical properties of the GNFs were dependent on the thickness of the Au coating layer and the degree of Ag dealloying. The optimal GNF exhibited a robust spherical skeleton composed of a few thick rims, but preserved the distinctive LSPR absorbance in the NIR region-even when the Ag content within the skeleton was only 10 wt %, 4-fold lower than that of the GNSs. These GNFs displayed an attractive photothermal conversion ability and great photothermal stability, and could efficiently kill 4T1 cancer cells through light-induced heating. Moreover, the GNFs preserved their morphology and optical properties after incubation in biological media (e.g., saline, serum), whereas the GNSs were unstable under the same conditions because of rapid dissolution of the considerable silver content with the shell. Furthermore, the GNFs had good biocompatibility with normal cells (e.g., NIH-3T3 and hepatocytes; cell viability for both cells: >90%), whereas the GNSs exhibited significant dose-dependent cytotoxicity (e.g., cell viability for hepatocytes at 1.14 nM: ca. 11%), accompanied by the induction of reactive oxygen species. Finally, the GNFs displayed good biocompatibility and biosafety in an in vivo mouse model; in contrast, the accumulation of GNSs caused liver injury and inflammation. Our results suggest that GNFs have great potential to serve as stable, biocompatible NIR-light absorbers for in vivo applications, including cancer detection and combination therapy.