Measurement of H2'-C2' and H3'-C3' dipolar couplings in RNA molecules.
Measurement of H2'-C2' and H3'-C3' dipolar couplings in RNA molecules.
复制标题
RNA 分子中 H2-C2 和 H3-C3 偶极耦合的测量。
DOI：
10.1023/a:1020620016891
复制
发表时间：
2002
影响因子：
2.7
通讯作者：
Moore,PeterB
中科院分区：
文献类型：
--
作者：
Vallurupalli,Pramodh;Moore,PeterB
The quality of nucleic acid solution structures can be significantly improved using residual dipolar coupling data. However, many of the one-bond couplings that could be used for this purpose are difficult to measure. Conventional 2D experiments are often unable to reveal one-bond H2′-C2′ and H3′-C3′ couplings in large RNA molecules due to spectral overlap. Here we show how to use 3D HCcH-COSY and Relay HCcH-COSY to measure one-bond H2′-C2′ and H3′-C3′ couplings which improved the precision of the structures obtained recently for a 42 nucleotide RNA.
