Modulation of pathogenicity with norepinephrine related to the type III secretion system of Vibrio parahaemolyticus
Modulation of pathogenicity with norepinephrine related to the type III secretion system of Vibrio parahaemolyticus
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DOI：
10.1086/513275
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发表时间：
2007-05-01
影响因子：
6.4
通讯作者：
Nakaya, Yutaka
中科院分区：
文献类型：
--
作者：
Nakano, Masayuki;Takahashi, Akira;Nakaya, Yutaka
背景去甲肾上腺素（NE）控制胃肠道的功能，但其在肠道病原菌致病中的作用尚不清楚。我们研究了NE对副溶血性弧菌III型分泌系统（TTSS）致病性的影响。为了评价NE对副溶血性弧菌致病性的影响，我们使用大鼠回肠袢模型检测了NE对Caco-2细胞的细胞毒活性和肠毒性。在动物回肠袢模型中，TTSS 1引起细胞毒性，TTSS 2引起肠毒性。我们的研究结果表明，虽然NE单独不影响Caco-2细胞的活力，NE刺激副溶血性弧菌的细胞毒活性。此外，NE增加TTSS 1相关基因vscQ和vscU的转录。这些结果表明NE调节副溶血性弧菌的细胞毒活性。NE通过与α 1-肾上腺素能受体相互作用而增强副溶血性弧菌的肠毒性。这些结果表明，肠上皮细胞上的α 1肾上腺素能受体似乎与副溶血性弧菌的肠毒性相互作用。目前的研究结果表明，肠道NE可能调节副溶血性弧菌的致病性。
Background. Norepinephrine (NE) controls the functions of the gastrointestinal tract, but its role in the pathogenicity of enteropathogens is not clear. We examined the effect of NE on the pathogenicity of Vibrio parahaemolyticus with regard to its type III secretion systems (TTSSs).Methods. To evaluate the effect of NE on pathogenicity of V. parahaemolyticus, we examined the cytotoxic activity to Caco-2 cells and enterotoxicity by use of the rat ileal loop model. It has been reported that TTSS1 causes cytotoxicity and that TTSS2 causes enterotoxicity in the animal ileal loop model.Results. Our results showed that, although NE alone did not affect the viability of Caco-2 cells, NE stimulated the cytotoxic activity of V. parahaemolyticus. Furthermore, NE increased the transcription of the TTSS1-related genes vscQ and vscU. These results indicate that NE regulates V. parahaemolyticus cytotoxic activity. The enterotoxicity of V. parahaemolyticus was increased by NE through interaction with a 1-adrenergic receptors. These results indicate that a 1-adrenergic receptors on the intestinal epithelium appear to interact with V. parahaemolyticus enterotoxicity.Conclusions. The present findings suggest that enteric NE may modulate V. parahaemolyticus pathogenicity.