CYCLIN IS DEGRADED BY THE UBIQUITIN PATHWAY
CYCLIN IS DEGRADED BY THE UBIQUITIN PATHWAY
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DOI：
10.1038/349132a0
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发表时间：
1991-01-10
期刊：
影响因子：
64.8
通讯作者：
KIRSCHNER, MW
中科院分区：
文献类型：
--
作者：
GLOTZER, M;MURRAY, AW;KIRSCHNER, MW
细胞周期蛋白降解是决定退出有丝分裂并进入下一个细胞周期的关键步骤。 当细胞周期蛋白N末端的一个区域与外源蛋白融合时，它产生一种在有丝分裂时易受蛋白水解的杂合蛋白。 在降解过程中，细胞周期蛋白和杂合物都与泛素形成缀合物。共轭物的动力学性质表明，细胞周期蛋白降解的泛素依赖的蛋白水解。 因此，细胞分裂后期可能是由泛素结合系统识别细胞周期蛋白而触发的。
Cyclin degradation is the key step governing exit from mitosis and progress into the next cell cycle. When a region in the N terminus of cyclin is fused to a foreign protein, it produces a hybrid protein susceptible to proteolysis at mitosis. During the course of degradation, both cyclin and the hybrid form conjugates with ubiquitin. The kinetic properties of the conjugates indicate that cyclin is degraded by ubiquitin-dependent proteolysis. Thus anaphase may be triggered by the recognition of cyclin by the ubiquitin-conjugating system.