Widespread thalamic terminations of fibers arising in the superficial medullary dorsal horn of monkeys and their relation to calbindin immunoreactivity
Widespread thalamic terminations of fibers arising in the superficial medullary dorsal horn of monkeys and their relation to calbindin immunoreactivity
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DOI：
10.1523/jneurosci.4122-03.2004
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发表时间：
2004-01-07
影响因子：
5.3
通讯作者：
Jones, EG
中科院分区：
文献类型：
--
作者：
Graziano, A;Jones, EG
来自丘脑脊髓和髓质背角的疼痛纤维的传递已成为一个有争议的问题。这项研究分析了 I 层中产生的纤维与腹侧后核复合体尾极内部和周围的核的关系，特别是与钙结合蛋白致密免疫反应区 (VMpo) 的关系，一些作者将其确定为这些纤维的唯一丘脑中继。我们表明，钙结合蛋白免疫反应性最密集的区域是更广泛的钙结合蛋白免疫反应区域的一部分，该区域位于腹侧后内侧核（VPM）的内侧尖端内，如其他染色方法所描绘的那样，并证明使用不同的抗钙结合蛋白抗体不能解释后丘脑组织解释的差异 地区。通过将免疫细胞化学染色与涉及层 I 的注射的顺行追踪相结合，我们证明了广泛的纤维末端，这些末端不限于 VPM 富含钙结合蛋白的内侧尖端，并且表明层 I 产生的纤维本身不具有钙结合蛋白免疫反应性。这项研究反驳了 VMpo 作为独立丘脑疼痛核或作为上行疼痛系统中特定中继的存在。
The relay of pain fibers from the spinal and medullary dorsal horn in the thalamus has become a controversial issue. This study analyzed the relationship of fibers arising in lamina I to nuclei in and around the caudal pole of the ventral posterior nuclear complex and especially to a zone of calbindin-dense immunoreactivity(VMpo) identified by some authors as the sole thalamic relay for these fibers. We show that the densest zone of calbindin immunoreactivity is part of a more extensive, calbindin-immunoreactive region that lies well within the medial tip of the ventral posterior medial nucleus (VPM), as delineated by other staining methods, and prove that the use of different anti-calbindin antibodies cannot account for differences in interpretations of the organization of the posterior thalamic region. By combining immunocytochemical staining with anterograde tracing from injections involving lamina I, we demonstrate widespread fiber terminations that are not restricted to the calbindin-rich medial tip of VPM and show that the lamina I arising fibers are not themselves calbindin immunoreactive. This study disproves the existence of VMpo as an independent thalamic pain nucleus or as a specific relay in the ascending pain system.