Evolutionary discrimination of mammalian conserved non-genic sequences (CNGs)
Evolutionary discrimination of mammalian conserved non-genic sequences (CNGs)
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DOI：
10.1126/science.1087047
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发表时间：
2003-11-07
期刊：
影响因子：
56.9
通讯作者：
Antonarakis, SE
中科院分区：
文献类型：
--
作者：
Dermitzakis, ET;Reymond, A;Antonarakis, SE
对人类和小鼠基因组的分析鉴定了大量保守的非基因序列（CNG）。它们的保存的意义和进化深度仍然没有答案。我们已经量化了14种哺乳动物中人类21号染色体的191个CNG的保守水平和模式。我们发现，CNG是显着更保守的比蛋白质编码基因和非编码RNA（ncRNA）的哺乳动物类从灵长类动物的单孔目动物有袋动物。CNG中的取代模式与蛋白质编码和ncRNA基因中的取代模式不同，与蛋白质结合区的取代模式相似。大约0.3%到1%的人类基因组对应于哺乳动物之间共享的一种以前未知的极度受限的CNG。
Analysis of the human and mouse genomes identified an abundance of conserved non-genic sequences (CNGs). The significance and evolutionary depth of their conservation remain unanswered. We have quantified levels and patterns of conservation of 191 CNGs of human chromosome 21 in 14 mammalian species. We found that CNGs are significantly more conserved than protein-coding genes and noncoding RNAS (ncRNAs) within the mammalian class from primates to monotremes to marsupials. The pattern of substitutions in CNGs differed from that seen in protein-coding and ncRNA genes and resembled that of protein-binding regions. About 0.3% to 1% of the human genome corresponds to a previously unknown class of extremely constrained CNGs shared among mammals.