Comparison of the acute effects of benzo-a-pyrene on adult zebrafish (Danio rerio) cardiorespiratory function following intraperitoneal injection versus aqueous exposure
Comparison of the acute effects of benzo-a-pyrene on adult zebrafish (Danio rerio) cardiorespiratory function following intraperitoneal injection versus aqueous exposure
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DOI：
10.1016/j.aquatox.2015.05.008
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发表时间：
2015-08-01
影响因子：
4.5
通讯作者：
Weber, Lynn P.
中科院分区：
文献类型：
--
作者：
Gerger, Courtney J.;Weber, Lynn P.
多环芳烃是普遍存在的环境污染物。接触多环芳烃会对多种鱼类造成发育毒性，而对成鱼的急性毒性被认为是最小的。文献越来越多地表明可能发生亚致死性PAH效应，但暴露途径的差异可能会混淆结论。我们假设成鱼急性接触多环芳烃将导致心肺功能损害，这种损害不会因接触途径而有所不同。为了研究这一假设，将成年斑马鱼（Danio rerio）腹膜内（i. p.）两次增加浓度的原型PAH，苯并-a-芘（BaP; 0.1，10，和1000 μ g/kg）或暴露于水（静态，更新在24小时; 16.2和162 μ g/L）48小时，并与相应的二甲亚砜对照。在任何接触浓度或途径下，均未发现鱼类死亡或对体重产生重大影响。在48小时时，对鱼进行游泳试验，同时进行耗氧量测量（n =10条鱼/处理）或超声心动图（n =12条鱼/处理）。与对照组相比，BaP注射组在三种游泳速度下的耗氧量（MO 2）增加（在双因素ANOVA后的Fisher's LSD检验中p < 0.01）。相比之下，水BaP暴露的鱼表现出增加MO 2仅在基础条件下。尽管增加了氧气需求，心室心率显着降低BaP暴露的鱼，无论是注射和水性暴露。通过对鱼类组织中苯并（a）芘体内负荷的分析，可以确定接触途径之间的重叠剂量组，然后通过比较心肺毒性。该比较显示，两种暴露途径之间的大多数影响相似，尽管注意到微小差异。在类似的BaP身体负担，注射鱼遭受更严重的心动过缓比水暴露的鱼，并有更大的水平增加细胞色素P4501 A（CYP 1A）的mRNA水平在肝脏和心脏组织相比，水暴露的鱼。总之，急性苯并（a）芘暴露对成年斑马鱼的心肺功能有负面影响。暴露途径之间的影响差异主要归因于生物利用度的差异，因为总体而言，当达到相似的BaP身体负荷时，在两种暴露途径之间观察到相似的影响。(C)2015 Elsevier B. V.版权所有。
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants. PAH exposure causes developmental toxicity in multiple fish species, while acute adult fish toxicity is thought to be minimal. The literature increasingly suggests sublethal PAH effects may occur, but differences in exposure route may confound conclusions. We hypothesized that acute PAH exposure in adult fish will cause cardiorespiratory impairment that will not differ with exposure route. In order to investigate this hypothesis, adult zebrafish (Danio rerio) were injected intraperitoneal (i.p.) twice with increasing concentrations of the prototypical PAH, benzo-a-pyrene (BaP; 0.1, 10, and 1000 mu g/kg) or exposed aqueously (static, renewal at 24 h; 16.2 and 162 mu g/L) for 48 h and compared to corresponding dimethylsulfoxide controls. No mortalities or significant effects on weight of the fish were noted at any exposure concentration or route. At 48 h, fish were subjected to swimming tests with concurrent oxygen consumption measurement (n =10 fish/treatment) or echocardiography (n =12 fish/treatment). Oxygen consumption (MO2) was increased at three swimming speeds in BaP-injected groups compared to control (p < 0.01 in Fisher's LSD tests after two-way ANOVA). In contrast, aqueously BaP-exposed fish showed increased MO2 under only basal conditions. Despite increased oxygen demand, ventricular heart rate was significantly decreased in BaP-exposed fish, both injected and aqueously-exposed. Analysis of BaP body burdens in fish tissue allowed for identification of an overlapping dose group between exposure routes, through which comparisons of cardiorespiratory toxicity were then made. This comparison revealed most effects were similar between the two exposures routes, although minor differences were noted. At similar BaP body burdens, injected fish suffered from more severe bradycardia than aqueously exposed fish and had greater levels of increases in cytochrome P4501A (CYP1A) mRNA levels in liver and heart tissue compared to aqueous exposed fish. In conclusion, acute BaP exposure in adult zebrafish had negative effects on cardiorespiratory function. Differences in effect between exposure routes were attributed primarily to differences in bioavailability, since overall, similar effects were noted between the two exposure routes when similar BaP body burdens were achieved. (C) 2015 Elsevier B.V. All rights reserved.