The MADS-domain factor AGAMOUS-Like18 promotes somatic embryogenesis
The MADS-domain factor AGAMOUS-Like18 promotes somatic embryogenesis
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DOI：
10.1093/plphys/kiab553
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发表时间：
2021-12-04
期刊：
影响因子：
7.4
通讯作者：
Perry, Sharyn E.
中科院分区：
文献类型：
--
作者：
Paul, Priyanka;Joshi, Sanjay;Perry, Sharyn E.
MADS-因子AGAMOUS-Like18(AGL18)与AGL15在物理和遗传上相互作用，促进体细胞胚胎发生，是植物再生的重要途径。AGL18是一种与AGL15结构上相关的MADS结构域转录因子。我们发现，与AGL15一样，AGL18在拟南芥中异位表达时可以促进体细胞胚胎发生(SE)。基于功能缺失突变体，AGL15和AGL18在SE等发育过程中具有冗余功能。为了了解这种冗余的性质，我们进行了一些研究，以考察这些因素之间的相互作用。我们利用染色质免疫沉淀(ChIP)-SEQ和RNA-SEQ相结合的方法研究了AGL18的全基因组直接靶点，以在分子水平上表征其功能。结果表明，AGL18可与基因组中的数千个位点结合。比较AGL15和AGL18的芯片序列数据发现，AGL15和AGL18都结合了大量的基因，但也存在差异。基因本体论分析表明，目标基因在种子、胚胎、生殖发育以及激素和胁迫反应中都得到了丰富。结果还表明，AGL15和AGL18在一个复杂的调控环中相互作用，其中AGL15抑制AGL18的转录积累，而AGL18促进AGL15的转录积累。免疫共沉淀显示AGL18和AGL15在体细胞胚胎组织中存在相互作用。结合和表达分析揭示了胚胎转录因子与它们的靶基因之间存在复杂的串扰和相互作用。此外，我们的研究还揭示了AGL18和AGL15的磷酸化对SE的促进至关重要。
The MADS-factor AGAMOUS-Like18 (AGL18) interacts physically and genetically with AGL15 to promote somatic embryogenesis, an important means of plant regeneration.AGAMOUS-Like 18 (AGL18) is a MADS domain transcription factor (TF) that is structurally related to AGL15. Here we show that, like AGL15, AGL18 can promote somatic embryogenesis (SE) when ectopically expressed in Arabidopsis (Arabidopsis thaliana). Based on loss-of-function mutants, AGL15 and AGL18 have redundant functions in developmental processes such as SE. To understand the nature of this redundancy, we undertook a number of studies to look at the interaction between these factors. We studied the genome-wide direct targets of AGL18 to characterize its roles at the molecular level using chromatin immunoprecipitation (ChIP)-SEQ combined with RNA-SEQ. The results demonstrated that AGL18 binds to thousands of sites in the genome. Comparison of ChIP-SEQ data for AGL15 and AGL18 revealed substantial numbers of genes bound by both AGL15 and AGL18, but there were also differences. Gene ontology analysis revealed that target genes were enriched for seed, embryo, and reproductive development as well as hormone and stress responses. The results also demonstrated that AGL15 and AGL18 interact in a complex regulatory loop, where AGL15 inhibited transcript accumulation of AGL18, while AGL18 increased AGL15 transcript accumulation. Co-immunoprecipitation revealed an interaction between AGL18 and AGL15 in somatic embryo tissue. The binding and expression analyses revealed a complex crosstalk and interactions among embryo TFs and their target genes. In addition, our study also revealed that phosphorylation of AGL18 and AGL15 was crucial for the promotion of SE.