Peroxidase-mediated oxidation, a possible pathway for metabolic activation of diethylstilbestrol.
Peroxidase-mediated oxidation, a possible pathway for metabolic activation of diethylstilbestrol.
复制标题
过氧化物酶介导的氧化，己烯雌酚代谢活化的可能途径。
DOI：
10.1016/0006-291x(78)90625-3
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发表时间：
1978
影响因子：
3.1
通讯作者：
J. Mclachlan
中科院分区：
文献类型：
--
作者：
M. Metzler;J. Mclachlan
从马萝卜或小鼠子宫中提取的过氧化物酶制剂在过氧化氢的存在下体外氧化己烯雌酚（DES）产生β-二烯雌酚，这也是一些哺乳动物体内DES的主要代谢物。氧化反应似乎涉及活性中间体，可能是DES的半醌和醌，因为发现了与鲑鱼精子脱氧核糖核酸和牛血清白蛋白不可提取的结合。过氧化物酶催化DES在雌激素靶器官氧化为活性代谢物，可能与这种合成雌激素的器官毒性有关。
In vitro oxidation of diethylstilbestrol (DES) by peroxidase preparations from horse radish or mouse uterus in the presence of hydrogen peroxide yields β-dienestrol, which is also a major in vivo metabolite of DES in several mammalian species. The oxidation reaction appears to involve reactive intermediates, presumably the semiquinone and quinone of DES, since nonextractable binding to salmon sperm deoxyribonucleic acid and bovine serum albumin was found. The peroxidase-catalyzed oxidation of DES to reactive metabolites in estrogen target organs may be related to the organ toxicity of this synthetic estrogen.