BENDING AND STRAIGHTENING OF DNA INDUCED BY THE SAME LIGAND - CHARACTERIZATION WITH THE ATOMIC-FORCE MICROSCOPE
BENDING AND STRAIGHTENING OF DNA INDUCED BY THE SAME LIGAND - CHARACTERIZATION WITH THE ATOMIC-FORCE MICROSCOPE
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DOI：
10.1021/bi00194a007
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发表时间：
1994-07-19
期刊：
影响因子：
2.9
通讯作者：
BRUICE, TC
中科院分区：
文献类型：
--
作者：
HANSMA, HG;BROWNE, KA;BRUICE, TC
一种新的配体 MGT-6b 通过两个连接部分与 DNA 结合：结合磷酸主链的多胺和结合小沟的三吡咯肽。该配体降低了弯曲动质体 DNA (kDNA) 的曲率，并且还增加了用作分子量标准 (M400) 的 400 bp DNA 的曲率，如原子力显微镜 (AFM) 所表征。 MGT-6b 在拉直 kDNA 方面比偏端霉素更有效。 MGT-6b 会延迟 M400 DNA 的电泳迁移率，尽管它的两个组成部分都不会改变 M400 的迁移率或其在 AFM 中看到的曲率。因此，影响 DNA 弯曲的“虎钳夹紧”结合模式需要配体的两个部分。 MGT-6b 弯曲和拉直 DNA 的能力可能与非常不同的 DNA 序列以及 kDNA 和 M400 DNA 的结构有关。
A new ligand, MGT-6b, binds to DNA with two linked parts: a polyamine that binds to the phosphate backbone and a tripyrrole peptide that binds to the minor groove. This ligand decreases the curvature of bent kinetoplast DNA (kDNA) and also increases the curvature of a 400-bp DNA that is used as a molecular weight standard (M400), as characterized with the atomic force microscope (AFM). MGT-6b is more effective than distamycin at straightening kDNA. MGT-6b retards the electrophoretic mobility of M400 DNA, although neither of its two component parts alters the mobility of M400 or its curvature as seen in the AFM. Thus, both parts of the ligand are needed for the ''vise grip'' mode of binding that affects DNA bending. The ability of MGT-6b to both bend and straighten DNA is probably related to the very different DNA sequences and, hence, structures of kDNA and M400 DNA.