MOLECULAR-CLONING OF MEI-41, A GENE THAT INFLUENCES BOTH SOMATIC AND GERMLINE CHROMOSOME METABOLISM OF DROSOPHILA-MELANOGASTER
MOLECULAR-CLONING OF MEI-41, A GENE THAT INFLUENCES BOTH SOMATIC AND GERMLINE CHROMOSOME METABOLISM OF DROSOPHILA-MELANOGASTER
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DOI：
10.1007/bf00294677
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发表时间：
1995-01-20
期刊：
影响因子：
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通讯作者：
BOYD, JB
中科院分区：
文献类型：
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作者：
BANGA, SS;YAMAMOTO, AH;BOYD, JB
黑腹果蝇的mei-41基因在减数分裂、维持体细胞染色体稳定性、复制后修复和DNA双链断裂修复中起着重要作用。利用可利用的染色体畸变，该基因在细胞遗传学上定位于多烯染色体带14C4-6。在细胞遗传间隔14C1-6的双向染色体行走后，分离出约60 kb的DNA序列。在那次行走中发现的染色体畸变断点确定了整个mei-41基因已被克隆。两个独立衍生的mei-41突变体已被证明在步行的单个2.2 kb片段中携带P插入。由于这些突变体的复生体已经失去了P元素序列，因此mei-41基因的一个重要区域存在于该片段中。一个横跨P插入位点的10.5 kb基因组片段被发现可以恢复mei-41（D3）突变体的甲磺酸抗性和雌性生育能力。结果表明，在该基因组片段上存在mei-41基因正确表达所需的所有序列。本研究为染色体稳定性在生殖细胞和体细胞中至关重要的功能的分子分析提供了基础。
The mei-41 gene of Drosophila melanogaster plays an essential role in meiosis, in the maintenance of somatic chromosome stability, in postreplication repair and in DNA double-strand break repair. This gene has been cytogenetically localized to polytene chromosome bands 14C4-6 using available chromosomal aberrations. About 60 kb of DNA sequence has been isolated following a bidirectional chromosomal walk that extends over the cytogenetic interval 14C1-6. The break-points of chromosomal aberrations identified within that walk establish that the entire mei-41 gene has been cloned. Two independently derived mei-41 mutants have been shown to carry P insertions within a single 2.2 kb fragment of the walk. Since revertants of those mutants have lost the P element sequences, an essential region of the mei-41 gene is present in that fragment. A 10.5 kb genomic fragment that spans the P insertion sites has been found to restore methyl methanesulfonate resistance and female fertility of the mei-41(D3) mutants. The results demonstrate that all the sequences required for the proper expression of the mei-41 gene are present on this genomic fragment. This study provides the foundation for molecular analysis of a function that is essential for chromosome stability in both the germline and somatic cells.