Donor-Derived Mesenchymal Stem Cells Remain Present and Functional in the Transplanted Human Heart
Donor-Derived Mesenchymal Stem Cells Remain Present and Functional in the Transplanted Human Heart
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DOI：
10.1111/j.1600-6143.2008.02450.x
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发表时间：
2009-01-01
影响因子：
8.8
通讯作者：
Baan, C. C.
中科院分区：
文献类型：
--
作者：
Hoogduijn, M. J.;Crop, M. J.;Baan, C. C.
间充质干细胞（MSC）的特点是其多谱系分化能力和免疫抑制特性。它们几乎存在于所有组织中，我们最近对来自人类心脏的 MSC 进行了表征。临床心脏移植为研究移植的人类间充质干细胞的命运提供了一个模型。在这项研究中，我们从心脏移植前以及心脏移植后 1 周至 6 年采集的心脏组织中分离并扩增了 MSC。移植后组织中的 MSC 均来自供体，这证明了内源性 MSC 的寿命长，并表明受体 MSC 没有迁移到心脏中。从移植组织中分离出的 MSC 显示出 MSC 特有的免疫表型，并保持了心肌生成和成骨分化能力。此外，它们还保留了抑制供体刺激的受体外周血单核细胞增殖反应的能力。总之，来自供体的功能性 MSC 在移植后仍存在于心脏中数年。
Mesenchymal stem cells (MSC) are characterized by their multilineage differentiation capacity and immunosuppressive properties. They are resident in virtually all tissues and we have recently characterized MSC from the human heart. Clinical heart transplantation offers a model to study the fate of transplanted human MSC. In this study, we isolated and expanded MSC from heart tissue taken before, and 1 week up to 6 years after heart transplantation. MSC from posttransplantation tissue were all of donor origin, demonstrating the longevity of endogenous MSC and suggesting an absence of immigration of recipient MSC into the heart. MSC isolated from transplanted tissue showed an immunophenotype that was characteristic for MSC and maintained cardiomyogenic and osteogenic differentiation capacity. They furthermore preserved their ability to inhibit the proliferative response of donor-stimulated recipient peripheral blood mononuclear cells. In conclusion, functional MSC of donor origin remain present in the heart for several years after transplantation.