X-ray diffraction from intact tau aggregates in human brain tissue.
X-ray diffraction from intact tau aggregates in human brain tissue.
复制标题
人脑组织中完整 tau 蛋白聚集体的 X 射线衍射。
DOI：
10.1016/j.nima.2011.01.059
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Rice,SarahE
中科院分区：
文献类型：
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作者：
Landahl,EricC;Antipova,Olga;Bongaarts,Angela;Barrea,Raul;Berry,Robert;Binder,LesterI;Irving,Thomas;Orgel,Joseph;Vana,Laurel;Rice,SarahE
We describe an instrument to record X-ray diffraction patterns from diseased regions of human brain tissue by combining an in-line visible light fluorescence microscope with an X-ray diffraction microprobe. We use thiazine red fluorescence to specifically label and detect the filamentous tau protein pathology associated with Pick's disease, as several laboratories have done previously. We demonstrate that thiazine red-enhanced regions within the tissue show periodic structure in X-ray diffraction, which is not observed in healthy tissue. One observed periodicity (4.2Å) is characteristic of cross-beta sheet structure, consistent with previous results from powder diffraction studies performed on purified, dried tau protein.
