Increase in the Nitrogen Content of Soil by the Introduction of Earthworms into Soil
Increase in the Nitrogen Content of Soil by the Introduction of Earthworms into Soil
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DOI：
10.1111/j.1747-0765.2005.tb00128.x
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发表时间：
2005-10
影响因子：
2
通讯作者：
T. Ozawa;C. Risal;R. Yanagimoto
中科院分区：
文献类型：
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作者：
T. Ozawa;C. Risal;R. Yanagimoto
研究了蚯蚓（Amynthas vittatus）对土壤氮含量增加的贡献。将三个成年蚯蚓标本放入装有 300 g 补充有 1% 羧甲基纤维素的土壤（灰色低地土壤、粉质粘土）的容器中，并在 22°C 下避光培养 32 d。蚯蚓孵化后土壤中全氮、NH4-N、NO3-N含量和好氧固氮菌数量显着增加，而土壤15N（δ15N）自然丰度下降。在微观世界的孵化过程中，蚯蚓体内的氮含量并没有减少。将蚯蚓引入土壤后，微观世界的乙炔还原活性和大气中 15N2 向土壤中 15N 的吸收均显着增强，尽管观察到的土壤中氮的增量远高于基于固氮活性估计的增量。本研究结果表明，蚯蚓增加了土壤的氮含量，这可能是由于蚯蚓从微观上增强了土壤的固氮活性。
The contribution of an earthworm species (Amynthas vittatus) to the increase of the nitrogen content of soil was examined. Three specimens of adult earthworms were introduced into 300 g of soil (Gray Lowland soil, silty clay) supplemented with 1% carboxymethyl cellulose in a container and incubated for 32 d at 22°C in the dark. The contents of total-N, NH4-N and NO3-N, and the population of aerobic nitrogen-fixing bacteria in soil significantly increased after incubation with the earthworms, while the natural abundance of 15N (δ15N) in soil decreased. The amount of nitrogen in the earthworms did not decrease during the incubation in the microcosm. Both acetylene reduction activity of the microcosm and incorporation of 15N to soil from atmospheric 15N2 were significantly enhanced by the introduction of the earthworms into soil, though the observed increment of nitrogen in soil was much higher than the estimated one based on the nitrogen-fixing activity. The results obtained in the present study indicated that the earthworms increased the nitrogen content of soil, presumably due to the enhancement of the nitrogen-fixing activity of the soil from the microcosm by the earthworms.