ANA, a novel member of Tob/BTG1 family, is expressed in the ventricular zone of the developing central nervous system
ANA, a novel member of Tob/BTG1 family, is expressed in the ventricular zone of the developing central nervous system
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DOI：
10.1038/sj.onc.1201805
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发表时间：
1998-05-21
期刊：
影响因子：
8
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Yoshida, Y;Matsuda, S;Yamamoto, T
使用聚合酶链反应介导的克隆程序，我们已经确定了一个新的成员，称为ANA（Abundant in Nueroepithelial Area）的抗增殖基因Tob/BTG 1家族的cDNA克隆和分析表明，人和小鼠ANA编码252个氨基酸的蛋白质，ANA的氨基端的一半与先前鉴定的抗增殖基因产物BTG 1同源，PC 3/TIS 21/BTG 2和Tob，人ANA基因定位于染色体21q11.2-q21.1，ANA在多种组织和细胞系中表达，其在卵巢、睾丸、前列腺、胸腺和肺中的表达较高。进一步的分析表明，ANA在发育中的中枢神经系统的脑室区高表达。最后，ANA的过表达损害了血清诱导的细胞周期从G（0）/G（1）期到S期的进程。总之，ANA是Tob/BTG 1家族的第四个成员，可能在中枢神经系统的神经发生中发挥作用。
Using a polymerase chain reaction-mediated cloning procedure, we have identified a novel member, termed ANA (from Abundant in Nueroepithelium Area), of Tob/BTG1 family of antiproliferative genes, Molecular cloning and analysis of cDNAs revealed that the human and mouse ANA encoded a protein of 252 amino acids, The amino-terminal half of ANA was homologous to the previously characterized antiproliferative gene products, BTG1, PC3/TIS21/BTG2, and Tob, The human, ANA gene was localized at chromosome 21q11.2-q21.1, ANA ,vas expressed in a variety of tissues and cell lines, its expression being high in the ovary, testis, prostate, thymus, and lung. Further analysis revealed that ANA expression was high in the ventricular zone of the developing central nervous system, Finally, overexpression of ANA impaired serum-induced cell cycle progression from the G(0)/G(1) to S phase. In conclusion, ANA is a fourth member of the Tob/BTG1 family that might play roles in neurogenesis in the central nervous system.