Poliovirus-binding inhibition ELISA based on specific chicken egg yolk antibodies as an alternative to the neutralization test
Poliovirus-binding inhibition ELISA based on specific chicken egg yolk antibodies as an alternative to the neutralization test
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DOI：
10.1016/j.jviromet.2019.01.007
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发表时间：
2019-04-01
影响因子：
3.1
通讯作者：
Ivanova, Olga E.
中科院分区：
文献类型：
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作者：
Ivanov, Alexander P.;Klebleyeva, Tatiana D.;Ivanova, Olga E.
本文介绍了应用鸡卵黄特异性抗体建立的酶免疫分析系统-结合抑制ELISA（BI ELISA）检测脊髓灰质炎病毒抗体的初步应用结果。ELISA的这种变体是“替代”中和试验（NT）。对90份口服脊髓灰质炎疫苗和灭活疫苗免疫儿童血清的抗体检测结果进行比较，BI ELISA法与NT法的相关性良好（1、2、3型分别为r = 0.67、0.61、0.76）。所介绍的ELISA变体是用于病毒感染（即脊髓灰质炎病毒感染）实验室诊断的“IgY技术”开发的进一步阶段，该技术可用于血清流行病学研究，作为NT的类似物，NT需要使用具有所需生物安全防护设施标准的活脊髓灰质炎病毒。
The first application results of enzyme immunoassay system (ELISA) - binding inhibition ELISA (BI ELISA) for the detection of antibodies to polioviruses of three types based on the use of specific antibodies from chicken yolk (IgY) are presented. This variant of ELISA is a "surrogate" neutralization test (NT). When comparing the results of the detection of antibodies in 90 sera of children who were vaccinated with oral and inactivated poliovirus vaccines, good correlation (r = 0.67, 0.61, 0.76 for types 1, 2, 3 respectively) between the BI ELISA and the NT results was shown. The presented variant of ELISA is the further stage of the development of "IgY-technology" for laboratory diagnostics of viral infections, namely poliovirus infection, which can be used for seroepidemiological studies as an analogue of the NT which requires the use of life poliovirus with the required standard of biosafety containment facilities.