SWITCH CIRCULAR DNA FORMED IN CYTOKINE-TREATED MOUSE SPLENOCYTES - EVIDENCE FOR INTRAMOLECULAR DNA DELETION IN IMMUNOGLOBULIN CLASS SWITCHING
SWITCH CIRCULAR DNA FORMED IN CYTOKINE-TREATED MOUSE SPLENOCYTES - EVIDENCE FOR INTRAMOLECULAR DNA DELETION IN IMMUNOGLOBULIN CLASS SWITCHING
复制标题
DOI：
10.1016/0092-8674(90)90247-c
复制
发表时间：
1990-07-13
期刊：
影响因子：
64.5
通讯作者：
SAKANO, H
中科院分区：
文献类型：
--
作者：
MATSUOKA, M;YOSHIDA, K;SAKANO, H
我们已经表征了用丝裂原脂多糖（LPS）和各种细胞因子（包括转化生长因子β）处理的小鼠脾细胞中的环状DNA。 (TGF-β)和白细胞介素4 (IL-4)。使用免疫球蛋白重链恒定基因（CH）的探针，鉴定了类别转换重组的切除产物。大多数克隆包含开关.mu的3''部分。 (S.mu.)区域和其他开关区域的5''部分。一些克隆含有3''-S.gamma。序列而不是3''-S.mu.。这表明同种型转换不仅可能发生在C.mu.，而且也可能发生在C.gamma.之一。基因与下游其他 CH 基因的连接。在 LPS 存在的情况下，细胞因子 TGF-β。增强了5''-S.α.阳性克隆的检测，而淋巴因子IL-4增强了5''-S.gamma.1阳性。数据支持 TGF-.beta 的观点。 IL-4可以指导同种型特异性类别转换。
We have characterized circular DNA in mouse splenocytes treated with the mitogen lipopolysaccharide (LPS) and various cytokines, including transforming growth factor .beta. (TGF-.beta.) and interleukin 4 (IL-4). Using probes of immunoglobulin heavy chain constant genes (CH), excision products of class switch recombination were identified. The majority of the clones contained the 3'' portion of the switch .mu. (S.mu.) region and the 5'' portion of other switch regions. Some clones contained 3''-S.gamma. sequences instead of 3''-S.mu.. This indicates that isotype switching may occur not only from C.mu., but also from one of the C.gamma. genes to other CH genes further downstream. In the presence of LPS, the cytokine TGF-.beta. enhanced the detection of 5''-S.alpha.-positive clones, while the lymphokine IL-4 enhanced 5''-S.gamma.1 positives. The data support the notion that TGF-.beta. and IL-4 can direct isotype-specific class switching.