Spectrum Fusion: Using Multiple Mass Spectra for De Novo Peptide Sequencing

Spectrum Fusion: Using Multiple Mass Spectra for De Novo Peptide Sequencing
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DOI:
10.1089/cmb.2009.0122
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发表时间:
2009-08-01
影响因子:
1.7
通讯作者:
Bern, Marshall
Bern, Marshall
中科院分区:
生物学4区
文献类型:
--
作者:
Datta, Ritendra;Bern, Marshall

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我们报告了一种新的算法,用于结合来自同一肽的几个质谱的信息。该算法自动学习肽片段化模式,因此它可以处理来自任何仪器和片段化技术的光谱。我们展示了该算法的实用性,以及多个光谱的能力,通过显示组合光谱对(一个CID和一个ETD)大大提高了从头测序的成功率。
We report on a new algorithm for combining the information from several mass spectra of the same peptide. The algorithm automatically learns peptide fragmentation patterns, so that it can handle spectra from any instrument and fragmentation technique. We demonstrate the utility of the algorithm, and the power of multiple spectra, by showing that combining pairs of spectra (one CID and one ETD) greatly improves de novo sequencing success rates.