Elongation factor Ts of Chlamydia trachomatis: structure of the gene and properties of the protein.
Elongation factor Ts of Chlamydia trachomatis: structure of the gene and properties of the protein.
复制标题
沙眼衣原体的延伸因子 Ts：基因结构和蛋白质特性。
DOI：
10.1006/abbi.1997.0178
复制
发表时间：
1997
影响因子：
3.9
通讯作者：
Miller,DL
中科院分区：
文献类型：
--
作者：
Zhang,Y;Tao,J;Zhou,M;Meng,Q;Zhang,L;Shen,L;Klein,R;Miller,DL
A putative structural gene cluster containing four open reading frames (ORFs) located downstream of theomp1gene ofChlamydia trachomatismouse pneumonitis (MoPn) was cloned and sequenced. A GenBank survey indicated that the identified cluster is similar to therpsB-tsf-pyrH(smbA)-frrregion ofEscherichia coli.The second ORF was 846 bp encoding a 282-amino-acid polypeptide with a calculatedMr30,824. Alignment of this deduced protein sequence andE. colielongation factor Ts (EF-Ts, product oftsf) demonstrated 34% identity and an additional 14% similarity. The putative chlamydialtsfgene was expressed inE. colias a nonfusion protein and as a 6× His-tagged fusion protein. By SDS–PAGE analysis, the molecular weights of the nonfusion recombinant protein and a protein of chlamydial elementary bodies (EBs), which was recognized by monoclonal antibodies derived from the nonfusion recombinant protein, are 34 kDa. The purified recombinant 6× His-tagged fusion protein increased the rate of GDP exchange with bothChlamydiaandE. colielongation factor Tu (EF-Tu). These data show that the second gene of the identified cluster istsf.Unlike EF-Ts from any other species, its activity was comparable to that ofE. coliEF-Ts in exchange reaction withE. coliEF-Tu.
