Effects of initial cell seeding in self assembly of articular cartilage.
Effects of initial cell seeding in self assembly of articular cartilage.
复制标题
初始细胞接种对关节软骨自组装的影响。
DOI：
10.1007/s10439-008-9524-x
复制
发表时间：
2008
影响因子：
3.8
通讯作者：
Athanasiou,KyriacosA
中科院分区：
文献类型：
--
作者：
Revell,ChristopherM;Reynolds,CatherineE;Athanasiou,KyriacosA
目前，利用自组装方法在体外进行关节软骨组织工程的研究已经产生了具有显著细胞外基质和由此产生的机械性能的结构。然而，大量的天然关节软骨细胞是产生功能性工程软骨所必需的;所有先前的自组装过程的工作都使用了5.5 × 106个细胞/构建体。在这项研究中，研究了初始细胞接种（0.25-11 × 106个细胞/构建体）对组织质量的影响。结果表明，当使用至少200万个细胞/构建体时，形成了组织工程化关节软骨，其具有接近天然组织的尺寸、组成和压缩特性。值得注意的是，更高的接种有助于更厚的结构和更大的直径，并对所得的生物化学和生物力学特性有显著影响。进一步观察到，聚集体模量随着接种量的增加而增加。结合大体形态学、组织学、生物化学和生物力学结果，确定了自组装过程的最佳初始接种量为3.75 × 106个细胞/构建体。这一发现通过将关节软骨组织工程所需的细胞数量减少32%，同时保持基本的组织特性，增强了这种组织工程过程的可转化性。
Current forays into tissue engineering of articular cartilagein vitrousing the self-assembling method have produced constructs possessing significant extracellular matrix and resulting mechanical properties. However, large numbers of native articular chondrocytes are necessary to produce functional engineered cartilage; all previous work with the self-assembling process has used 5.5 × 106cells/construct. In this study, the effects of initial cell seeding (0.25–11 × 106cells/construct) on tissue quality were investigated. Results showed that tissue engineered articular cartilage was formed, when using at least 2 million cells/construct, possessing dimensional, compositional, and compressive properties approaching those of native tissue. It was noted that higher seeding contributed to thicker constructs with larger diameters and had a significant effect on resulting biochemical and biomechanical properties. It was further observed that aggregate modulus increased with increased seeding. By combining gross morphological, histological, biochemical, and biomechanical results, an optimal initial seeding for the self-assembling process of 3.75 × 106cells/construct was identified. This finding enhances the translatability of this tissue engineering process by reducing the number of cells needed for tissue engineering of articular cartilage by 32% while maintaining essential tissue properties.