Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum vicaryi L. Fruit
Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum vicaryi L. Fruit
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女贞果多糖的免疫调节和抗氧化活性
DOI：
10.1155/2020/5431350
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发表时间：
2020-03-17
影响因子：
--
通讯作者：
Qin, Wei
中科院分区：
文献类型：
--
作者：
Liu, Shuling;Wang, Litao;Qin, Wei
金叶女贞是女贞的杂交种。变种金边女贞和女贞，属于木犀科。由于其金色的叶子，它经常被用作观赏灌木。然而，它的医学价值还有待发现。近年来，植物多糖因其具有免疫调节和抗氧化等生物学活性而受到广泛关注。对金叶女贞多糖进行提取、纯化及性质研究。研究其免疫调节和抗氧化活性。金叶女贞采用超声波提取、乙醇沉淀、大孔树脂分离、透析袋纯化等方法，得到水果多糖（LVFP）。采用傅里叶变换红外光谱法、高效离子色谱法和高效凝胶过滤色谱法对LVFP的理化性质进行了研究。结果表明，LVFP由鼠李糖、阿拉伯糖、半乳糖和葡萄糖组成，其组成比为1.79：7.55：4.58：1.54，分子量为88，949 Da。使用环磷酰胺（Cy-）诱导的免疫抑制小鼠模型研究了LVFP的免疫调节和抗氧化活性。结果表明，LVFP能显著提高脾脏和胸腺指数，增强中性粒细胞吞噬功能，激活B和T淋巴细胞，上调血清IL-10和TNF-α水平。此外，我们观察到LVFP减轻Cy-induced肝损伤，通过增加超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-px）的水平。结果表明，LVFP具有免疫调节和抗氧化活性，为LVFP在医药和功能食品工业中的应用奠定了基础。
Ligustrum vicaryi L. is a hybrid of Ligustrum ovalifolium Hassk. var. aureo-marginatum and Ligustrum vulgale L., belonging to the Oleaceae family. It is often used as an ornamental shrub due to its golden leaves. However, its medical value is yet to be discovered. Recently, plant polysaccharides have attracted comprehensive attention owing to their biological properties, including immunomodulatory and antioxidant activities. This study aimed to extract, purify, and characterize the polysaccharide from the Ligustrum vicaryi L. fruit and investigate its immunomodulatory and antioxidant activities. The Ligustrum vicaryi L. fruit polysaccharide (LVFP) was obtained by ultrasonic extraction, ethanol precipitation, macroporous resin separation, and dialysis bag purification. The physicochemical properties of the LVFP were elucidated using Fourier-transform infrared spectrometry, high-performance ion chromatography, and high-performance gel filtration chromatography. The results indicated that the LVFP consisted of rhamnose, arabinose, galactose, and glucose in a ratio of 1.79 : 7.55 : 4.58 : 1.54, and its molecular weight was 88,949 Da. The immunomodulatory and antioxidant activities of the LVFP were investigated using a cyclophosphamide- (Cy-) induced immunosuppressed mouse model. The results demonstrated that the LVFP significantly increased spleen and thymus indexes, enhanced the phagocytic function of neutrophils, activated B and T lymphocytes, and upregulated serum levels of IL-10 and TNF-α. Moreover, we observed that the LVFP relieved Cy-induced liver damage by increasing superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) levels. These results suggested that the LVFP has the immunomodulatory and antioxidant activities, therefore laying a foundation for the application of the LVFP in the pharmaceutical and functional food industries.