Characterization of sodium, potassium, ATPase activity in the gills of Pimephales promelas (fathead minnow): influence of in vitro exposure to lead.
Characterization of sodium, potassium, ATPase activity in the gills of Pimephales promelas (fathead minnow): influence of in vitro exposure to lead.
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Pimephales promelas（黑头米诺）鳃中钠、钾、ATP 酶活性的表征：体外接触铅的影响。
DOI：
10.1007/s00128-002-0074-x
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发表时间：
2002
影响因子：
2.7
通讯作者：
Cohen,GM
中科院分区：
文献类型：
--
作者：
Spokas,EG;Crivellone,MD;Kemp,F;Bogden,JD;Cohen,GM
本文重点关注水性铅 (Pb) 对鳃渗透调节功能的影响，考虑到这种重金属在天然水中的广泛分布，并且有证据表明 Pb 可能优先在鳃组织中积聚，因此特别令人感兴趣。通过线性回归分析确定无机磷酸盐管的纳摩尔数。每个测定中都包括底物空白。文章中显示了钠离子和钾离子刺激的 ATP 水解的平均速率，该速率是通过三项动力学测定（每项均使用来自单条鱼的鳃匀浆进行的）测定的。
This article focuses on the effects of water-borne lead (Pb) on gill osmoregulatory function is of particular interest considering the widespread distribution of this heavy metal in natural waters, and evidence suggesting that Pb may preferentially accumulate in gill tissue. Nanomoles of inorganic phosphate tube were determined by linear regression analysis. Included in each assay were substrate blanks. The mean rate of sodium ion and potassium ion-stimulated ATP hydrolysis, as determined in three kinetic assays each conducted using gill homogenate from a single fish, is shown in the article.