Toward Single Cell Tattoos: Biotransfer Printing of Lithographic Gold Nanopatterns on Live Cells
Toward Single Cell Tattoos: Biotransfer Printing of Lithographic Gold Nanopatterns on Live Cells
复制标题
迈向单细胞纹身：活细胞上平版金纳米图案的生物转印
DOI：
10.1021/acs.nanolett.3c01960
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发表时间：
2023
期刊：
影响因子：
10.8
通讯作者：
Gracias, David H.
中科院分区：
文献类型：
--
作者：
Kwok, Kam Sang;Zuo, Yi;Choi, Soo Jin;Pahapale, Gayatri J.;Gu, Luo;Gracias, David H.
光刻、电子束光刻和纳米压印光刻等光刻技术已经彻底改变了现代电子学和光学。然而，它们在制造纳米生物界面方面的应用受到传统制造方法的细胞毒性和二维性质的限制。在这里，我们提出了一种具有生物相容性和成本效益的转移过程，该过程利用(a) NIL定义低于300 nm的金（Au）纳米模式阵列，(b) Au的胺功能化将NIL阵列从刚性底物转移到软转移层，(c)海藻酸盐水凝胶作为柔性，可降解的转移层，以及(d) Au NIL阵列的明胶偶联以实现与活细胞的保形接触。我们展示了在大鼠大脑和活细胞上进行Au - nil阵列的生物转移打印，具有高模式保真度和细胞活力，并观察了Au - nil点和nil线打印水凝胶上细胞迁移的差异。我们预计这种纳米光刻兼容的生物转移打印方法可以促进仿生学，生物传感和生物杂交组织界面。
Lithographic nanopatterning techniques such as photolithography, electron-beam lithography, and nanoimprint lithography (NIL) have revolutionized modern-day electronics and optics. Yet, their application for creating nanobio interfaces is limited by the cytotoxic and two-dimensional nature of conventional fabrication methods. Here, we present a biocompatible and cost-effective transfer process that leverages (a) NIL to define sub-300 nm gold (Au) nanopattern arrays, (b) amine functionalization of Au to transfer the NIL-arrays from a rigid substrate to a soft transfer layer, (c) alginate hydrogel as a flexible, degradable transfer layer, and (d) gelatin conjugation of the Au NIL-arrays to achieve conformal contact with live cells. We demonstrate biotransfer printing of the Au NIL-arrays on rat brains and live cells with high pattern fidelity and cell viability and observed differences in cell migration on the Au NIL-dot and NIL-wire printed hydrogels. We anticipate that this nanolithography-compatible biotransfer printing method could advance bionics, biosensing, and biohybrid tissue interfaces.