Protection of BALB/c mice against Brucella melitensis 16 M infection induced by vaccination with live Escherchia coli expression Brucella P39 protein
Protection of BALB/c mice against Brucella melitensis 16 M infection induced by vaccination with live Escherchia coli expression Brucella P39 protein
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DOI：
10.1016/j.vaccine.2009.12.012
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发表时间：
2010-02-17
期刊：
影响因子：
5.5
通讯作者：
Al-Mariri, Ayman
中科院分区：
文献类型：
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作者：
Al-Mariri, Ayman
羊种布氏杆菌16 M的周质结合蛋白（P39）抗原先前被鉴定为Th 1优势抗原。在这项研究中，这种抗原作为抗B疫苗的潜力。分析了BALB/c小鼠中羊种球蛋白16 M感染的情况，并对诱导的体液和细胞免疫应答进行了表征。小鼠腹腔内注射单独的活大肠杆菌或表达布鲁氏菌P39的大肠杆菌，间隔4周两次。活的E.大肠杆菌BL 21（DE 3）pEt 15 b-p39疫苗在用细菌提取物或作为特异性抗原的P39再刺激时引起T细胞增殖应答，并且还诱导γ干扰素产生。也是活的E。coli BL 21（DE 3）pEt 15 b-p39疫苗能诱导较强的体液免疫应答（IgG 1和IgG 2a）。与盐水接种的对照相比，在攻击感染前3周用大肠杆菌BL 21 pEt 15 b-p39接种小鼠，在所有接种的小鼠中，在攻击感染后4周和8周显著减少了脾脏中菌株16 M细菌的数量（P < 0.001）。(C)2009爱思唯尔有限公司版权所有
The periplasmic binding protein (P39) antigen of Brucella melitensis 16M was previously identified as Th1 dominant antigens. In this study, the potential for this antigen to function as vaccine against B. melitensis 16M infection in BALB/c mice has been analyzed, and the humoral and cellular immune responses induced have been also characterized. Mice were injected intraperitoneally with live Escherichia coli alone or with that which express Brucella P39, two times at 4 weeks intervals. The live E. coli BL21 (DE3) pEt15b-p39 vaccine elicited a T-cell-proliferative response and also induced a gamma interferon production upon re-stimulation with either the bacterial extract or P39 as a specific antigen. Also the live E. coli BL21 (DE3) pEt15b-p39 vaccine has been found to induce a strong humoral response (IgG1 and IgG2a). Compared to the saline-inoculated control, vaccination of mice with E coli BL21pEt15b-p39 at 3 weeks prior to the challenge infection, significantly reduced the number of strain 16 M bacteria in spleens at 4 and 8 weeks post-challenge infection in all vaccinated mice (P < 0.001). (C) 2009 Elsevier Ltd. All rights reserved