Effect of endurance exercise on myosin heavy chain isoform expression in diabetic rats with peripheral neuropathy.
Effect of endurance exercise on myosin heavy chain isoform expression in diabetic rats with peripheral neuropathy.
复制标题
耐力运动对糖尿病周围神经病变大鼠肌球蛋白重链亚型表达的影响。
DOI：
10.1097/01.phm.0000176350.61935.d6
复制
发表时间：
2005
影响因子：
3
通讯作者：
Thompson,LadoraV
中科院分区：
文献类型：
--
作者：
Snow,LeannM;Sanchez,OttoA;McLoon,LindaK;Serfass,RobertC;Thompson,LadoraV
张建军，张建军，张建军，张建军。耐力运动对糖尿病周围神经病变大鼠肌球蛋白重链亚型表达的影响。中华医学杂志2005；84: 770 - 779。目的：研究耐力运动对糖尿病周围神经病变大鼠比目鱼肌肌球蛋白重链（myosin heavy chain， MHC）异构体表达的影响。设计：雄性Sprague Dawley大鼠随机分为4组：对照组久坐组、糖尿病久坐组、对照组运动组和糖尿病运动组。锻炼后的动物每周在跑步机上跑五次。12周后，电生理测试显示糖尿病大鼠周围神经病变。然后切除比目鱼肌并快速冷冻。对横截面进行慢速、快速、发育和新生儿mhc的免疫组织化学染色。然后测定纤维类型组成和纤维横截面积。结果：无论运动状态如何，糖尿病组快速MHC百分比均显著高于对照组（糖尿病久坐22.6%；糖尿病运动25.2%；对照组久坐13.5%；对照组运动13.1%）。糖尿病患者的慢速MHC比例也明显低于对照组（糖尿病患者久坐，77.1%；糖尿病患者运动，74.3%；糖尿病患者久坐，86.2%；糖尿病患者运动，86.1%）。各组间肌纤维横截面积无显著差异。运动动物的发育性MHC表达高于不运动动物（糖尿病不运动，1.6%；糖尿病运动，3.8%；对照组不运动，0.8%；对照组运动，2.0%）。结论：糖尿病肌肉中慢速和快速MHC表达的改变与其他几种情况下的MHC表达相似，包括神经肌肉活动减少和去神经支配。这种MHC表达转移的机制尚不清楚。慢性耐力训练不改变成年糖尿病动物的MHC表达。发育性MHC表达可能是上坡跑步时比目鱼肌偏心收缩导致肌纤维损伤和再生的表现。
Snow LM, Sanchez OA, McLoon LK, Serfass RC, Thompson LV: Effect of endurance exercise on myosin heavy chain isoform expression in diabetic rats with peripheral neuropathy. Am J Phys Med Rehabil 2005; 84: 770–779.Objective:This study evaluated the effect of endurance exercise on myosin heavy chain (MHC) isoform expression in soleus muscle of diabetic rats with peripheral neuropathyDesign:Male Sprague Dawley rats were randomly divided into four groups: control sedentary, diabetic sedentary, control exercise, and diabetic exercise. The exercised animals performed treadmill running five times per week. After 12 wks, electrophysiologic testing documented peripheral neuropathy in the diabetic rats. The soleus muscles were then excised and quick-frozen. Cross-sections were immunohistochemically stained for slow, fast, developmental, and neonatal MHCs. Fiber-type composition and fiber cross-sectional areas were then determined.Results:The diabetic groups showed a significantly greater percentage of fast MHC than did the control groups, regardless of exercise status (diabetic sedentary, 22.6%; diabetic exercise, 25.2%; control sedentary, 13.5%; control exercise, 13.1%). The diabetics also showed a significantly lower percentage of slow-only MHC than controls (diabetic sedentary, 77.1%; diabetic exercise, 74.3%; control sedentary, 86.2%; control exercise, 86.1%). No differences in muscle fiber cross-sectional area existed between the groups. The exercised animals showed greater expression of developmental MHC than did the sedentary animals (diabetic sedentary, 1.6%; diabetic exercise, 3.8%; control sedentary, 0.8%; control exercise, 2.0%).Conclusion:The altered slow and fast MHC expression in the diabetic muscle is similar to MHC expression in several other conditions, including decreased neuromuscular activity and denervation. Mechanisms of this MHC expression shift are unknown. Chronic endurance training does not alter adult MHC expression in the diabetic animals. The developmental MHC expression is likely a manifestation of uphill treadmill running due to eccentric contractions in the soleus resulting in myofiber injury and regeneration.