EuNPs-MAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of fluorene
EuNPs-MAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of fluorene
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DOI：
10.1016/j.snb.2018.01.231
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发表时间：
2018-06-01
影响因子：
8.4
通讯作者：
Zhou, Yu
中科院分区：
文献类型：
--
作者：
Qiao, Bin;Li, Yansong;Zhou, Yu
荧烯是美国环境保护局列出的水中的优先污染物。合成了一种用单抗(MAb)标记Eu(III)标记的聚苯乙烯纳米粒子(EuNPs)的荧光探针，并建立了基于该探针的免疫层析分析方法(EuNPs-MAb)，用于快速、灵敏地检测水样中的荧烯。在0.98~62.50 ng/mL范围内，荧烯浓度与T线的荧光强度成反比。荧光定量检测的检出限为0.09 ng/mL。不同水样中荧烯的加标回收率为81.5%~103.00%，变异系数(CVs)均小于13%，与传统的酶联免疫吸附试验结果一致(R2=0.99)。检测可在30分钟内完成。集成的ICA试纸具有更高的灵敏度和可靠的重复性。该方法有望成为一种快速、灵敏的检测水样中荧烯的方法。(C)2018爱思唯尔B.V.保留所有权利。
Fluorene is a priority pollutant in water listed by U.S. Environmental Protection Agency. A fluorescent probe using Eu(III)-marked polystyrene nanoparticles (EuNPs) labelled with a monoclonal antibody (MAb) was synthesized and an immunochromatographic assay (ICA) based on the probe (EuNPs-MAb) was developed for rapid and sensitive detection of fluorene in water samples. The concentration of fluorene was inversely proportional to fluorescence intensity values of T-line in the range 0.98-62.50 ng/mL. The limit of detection (LOD) for quantitative detection of fluorene was 0.09 ng/mL. Recoveries of fluorene in various water samples ranged from 81.50% to 103.00% and coefficients of variation (CVs) were below 13%, which were in a good agreement with those of traditional ELISA (R-2 = 0.99). The assay can be performed within 30 min. The integrated ICA strip gave higher sensitivity and reliable reproducibility. It could be a potential method for rapid and sensitive detection of fluorene in water samples. (C) 2018 Elsevier B.V. All rights reserved.