Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv
Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv
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DOI：
10.1002/pmic.200600853
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发表时间：
2007-05-01
期刊：
影响因子：
3.4
通讯作者：
Wiker, Harald G.
中科院分区：
文献类型：
--
作者：
Malen, Hiwa;Berven, Frode S.;Wiker, Harald G.
结核分枝杆菌分泌的蛋白质在结核病的发病机制中起重要作用。对M.由Sadamu永井（日本）制造的结核病H37 Rv，与其它培养物相比，分泌蛋白显著富集。互补方法用于鉴定这些培养物中分泌的蛋白质：（i）2-DE结合MALDI-TOF MS和（ii）LC偶联MS/MS。鉴定了来自总共257种蛋白质的肽，其中144种通过一种以上的肽鉴定。发现免疫学上重要的早期分泌抗原靶标-6（ESAT-6）蛋白家族的几个成员是主要组分。大多数鉴定的蛋白质，159（62%），预计将通过一般分泌途径出口。我们实验证实了信号肽，介导易位。在41个已鉴定的蛋白质中，通过细胞膜，已经被去除，并且在其中35个蛋白质中，在切割位点的N-末端存在AXA基序，表明该基序对于分枝杆菌中信号肽的识别和切割是重要的。大部分的分泌蛋白是未知的，这表明我们已经绘制了一个未探索的部分输出蛋白质组的M。结核
Proteins secreted by Mycobacterium tuberculosis play an essential role in the pathogenesis of tuberculosis. The culture filtrates of M. tuberculosis H37Rv made by Sadamu Nagai (Japan), are considerably enriched for secreted proteins compared to other culture filtrates. Complementary approaches were used to identify the secreted proteins in these culture filtrates: (i) 2-DE combined with MALDI-TOF MS and (ii) LC coupled MS/MS. Peptides derived from a total of 257 proteins were identified of which 144 were identified by more than one peptide. Several members of the immunologically important early secretory antigenic target-6 (ESAT-6) family of proteins were found to be major components. The majority of the identified proteins, 159 (62%), were predicted to be exported through the general secretory pathway. We experimentally verified that the signal peptides, which mediate translocation. through the cell membrane, had been removed in 41 of the identified proteins, and in 35 of those, there was an AXA motif N-terminally to the cleavage site, showing that this motif is important for the recognition and cleavage of signal peptides in mycobacteria. A large fraction of the secreted proteins were unknown, suggesting that we have mapped an unexplored part of the exported proteome of M. tuberculosis.