CD24 Expression Is a Novel Prognostic Factor in Esophageal Squamous Cell Carcinoma
CD24 Expression Is a Novel Prognostic Factor in Esophageal Squamous Cell Carcinoma
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DOI：
10.1245/s10434-008-0252-0
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发表时间：
2009-02-01
影响因子：
3.7
通讯作者：
Kuwano, Hiroyuki
中科院分区：
文献类型：
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作者：
Sano, Akihiko;Kato, Hiroyuki;Kuwano, Hiroyuki
食管癌是最难治愈的恶性肿瘤之一，识别这种疾病患者的新预后标志物是重要的。CD24是一种高度糖基化的小粘蛋白样蛋白。一些研究表明，在各种恶性肿瘤中，较高的CD24表达与较短的患者生存期显著相关。然而，CD24在食管癌中的表达及其临床病理意义仍不清楚。应用免疫组织化学方法检测151例食管鳞癌组织中CD24和Ki-67的表达，探讨CD24表达与食管鳞癌临床病理参数及患者生存期的关系。对来源于食管癌的五种细胞系进行Western印迹分析以评估CD24表达。免疫组化显示CD24阳性61例（40.4%）。CD24表达与淋巴结转移（p = 0.005）、病理分期（p = 0.018）、淋巴结转移数目（p = 0.003）、淋巴管浸润（p = 0.002）、静脉浸润（p <0.001）和Ki-67标记指数（p <0.001）相关。CD24表达评分与Ki-67标记指数之间的Pearson相关系数为0.404（p <0.001）。CD24表达与无病生存率相关（p <0.001）。考克斯多元回归分析显示，CD 24表达是一个独立的预后因素（p = 0.033）。在Western印迹分析中，在所有五个细胞系中检测到CD24。我们的结论是，CD24的过度表达，这是与Ki-67的表达，是一种新的独立的预后指标，用于确定患者的预后不良后，食管鳞状细胞癌根治性切除术。
Esophageal cancer is one of the most difficult malignancies to cure, and identification of novel prognostic markers for patients with this disease is important. CD24 is a small highly glycosylated mucin-like protein. Several studies have shown that higher CD24 expression is significantly associated with shorter patient survival in various malignant tumors. However, the expression of CD24 and its clinicopathological significance in esophageal cancer remain largely unknown. Immunohistochemical analyses of CD24 and Ki-67 overexpression in 151 cases of esophageal squamous cell carcinoma were performed to examine the relationships of CD24 expression with clinicopathological parameters and patient survival. Five cell lines derived from esophageal cancer were subjected to Western blot analyses to evaluate CD24 expression. Immunohistochemically, CD24 expression was judged to be positive in 61 (40.4%) cases. CD24 expression was associated with lymph node metastasis (p = 0.005), pathologic stage (p = 0.018), number of nodal metastases (p = 0.003), lymphatic invasion (p = 0.002), venous invasion (p < 0.001), and Ki-67 labeling index (p < 0.001). The Pearson's correlation coefficient between the CD24 expression score and the Ki-67 labeling index was 0.404 (p < 0.001). CD24 expression was associated with disease-free survival (p < 0.001). A Cox multivariate regression analysis revealed that CD24 expression was an independent prognostic factor (p = 0.033). On Western blot analysis, CD24 was detected in all five cell lines. We conclude that overexpression of CD24, which was correlated with Ki-67 expression, is a novel independent prognostic marker for identifying patients with poor prognosis after curative resection of esophageal squamous cell carcinoma.