Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines
Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines
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DOI：
10.1002/jmv.24366
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发表时间：
2016-04-01
影响因子：
12.7
通讯作者：
Hu, Kanghong
中科院分区：
文献类型：
--
作者：
Cheng, Zhikui;Zhi, Xiaoguang;Hu, Kanghong
B型肝炎病毒（HBV）感染是世界范围内最严重和最普遍的健康问题之一。目前的抗HBV药物有许多缺点，如副作用和耐药性;因此，需要新的潜在的抗HBV试剂。硒（Se）已被证明与人类免疫缺陷病毒和丙型肝炎病毒感染有关，但其在HBV感染中的作用尚不清楚。为了解决这一问题，将亚硒酸钠（Na 2SeO 3）应用于三种HBV细胞模型：HepG2.2.15细胞和用1.1或1.3x HBV质粒转染的HuH-7细胞。采用Cell Counting Kit-8检测Na 2SeO 3的细胞毒性。分别用酶联免疫吸附试验、北方印迹法和南方印迹法测定病毒抗原表达、转录本和病毒DNA的水平。在HepG2.2.15或HuH-7细胞中，
Hepatitis B virus (HBV) infection is one of the most serious and prevalent health problems worldwide. Current anti-HBV medications have a number of drawbacks, such as adverse effects and drug resistance; thus, novel potential anti-HBV reagents are needed. Selenium (Se) has been shown to be involved in both human immunodeficiency virus and hepatitis C virus infections, but its role in HBV infection remains unclear. To address this, sodium selenite (Na2SeO3) was applied to three HBV cell models: HepG2.2.15 cells, and HuH-7 cells transfected with either 1.1 or 1.3x HBV plasmids. Cytotoxicity of Na2SeO3 was examined by Cell Counting Kit-8. Levels of viral antigen expression, transcripts, and encapsidated viral DNA were measured by enzyme-linked immunosorbent assay, northern blot, and Southern blot, respectively. There was no obvious cytotoxicity in either HepG2.2.15 or HuH-7 cells with