TBP-like protein (TLP) represses myogenesis via inhibition of the myogenin promoter.
TBP-like protein (TLP) represses myogenesis via inhibition of the myogenin promoter.
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TBP 样蛋白 (TLP) 通过抑制肌生成素启动子来抑制肌生成。
DOI：
10.1016/j.bbrc.2016.09.127
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Tamura T
中科院分区：
文献类型：
--
作者：
Nakazato A;Maeda R;Ishikawa K;Suzuki H;Tamura T
类TBP-like蛋白（TLP）是后生动物限制性转录因子之一，参与后生动物的发育和分化，但其调控机制尚不清楚。本研究探讨了TLP与小鼠C2 C12成肌细胞成肌作用的关系。我们发现，TLP基因的表达减少肌分化过程中。过度表达和敲低的TLP显示，肌肉特异性肌球蛋白重链和肌细胞生成转录因子myogenin的水平下调的TLP。TLP抑制从成肌细胞到肌管的形态变化的进展，从而抑制肌生成。我们进一步表明，TLP抑制肌细胞生成素的启动子活性。肌球蛋白启动子的近端富含AT的序列负责TLP介导的转录抑制。本研究结果表明，TLP通过下调myogeningene抑制肌生成。
TBP-like protein (TLP) is one of the metazoan-restricted transcription factors participating in development and differentiation, though the molecular mechanism by which TLP regulates these processes remains unclear. In this study, we investigated the relationship between TLP and myogenesis of mouse C2C12 myoblasts. We found that TLP gene expression decreases during myogenic differentiation. Overexpression and knockdown of TLP revealed that the levels of muscle-specific myosin heavy chain and the myogenic transcription factor myogenin are downregulated by TLP. TLP inhibits the progression of morphological change from myoblasts to myotubes, thereby suppressing myogenesis. We further show that TLP represses the promoter activity ofmyogenin. The proximal AT-rich sequence of themyogeninpromoter is responsible for TLP-mediated transcriptional repression. The results of this study suggest that TLP inhibits myogenesis through downregulation of themyogeningene.