CHARACTERIZATION OF ALEUTIAN DISEASE VIRUS AS A PARVOVIRUS
CHARACTERIZATION OF ALEUTIAN DISEASE VIRUS AS A PARVOVIRUS
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DOI：
10.1128/jvi.35.3.836-843.1980
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发表时间：
1980-01-01
影响因子：
5.4
通讯作者：
WOLFINBARGER, JB
中科院分区：
文献类型：
--
作者：
BLOOM, ME;RACE, RE;WOLFINBARGER, JB
阿留申病病毒株适应于 31.8°C 的 Crandall 猫肾细胞中生长。 C 被表征。当从受感染的细胞中纯化时，阿留申病病毒在 CsCl 中的密度为 1.42-1.44 g/ml，直径为 24-26 nm。 [3H]胸苷可以整合到病毒基因组中，然后研究病毒DNA。在碱性蔗糖梯度中，阿留申病病毒 DNA 是单一物种，在 15.5S 下与腺相关病毒的单链 DNA 共沉淀。当在中性蔗糖梯度上分析 DNA 时，再次观察到在 21S 处沉积的单一物种，并且与 16S 双链腺相关病毒 DNA 明显不同。即使在退火条件下温育后也获得了类似的结果，这意味着大部分阿留申病病毒病毒粒子含有MW为约1.4倍的单条非互补链。 106.通过对从用[35S]甲硫氨酸标记的感染培养物中纯化的病毒进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，证实了两种主要的病毒相关多肽，其MW为89,100和77,600。阿留申病病毒显然是一种无缺陷的细小病毒。
A strain of Aleutian disease virus adapted to growth in Crandall feline kidney cells at 31.8.degree. C was characterized. When purified from infected cells, Aleutian disease virus had a density in CsCl of 1.42-1.44 g/ml and was 24-26 nm in diameter. [3H]thymidine could be incorporated into the viral genome and the viral DNA was then studied. In alkaline sucrose gradients, Aleutian disease virus DNA was a single species that cosedimented at 15.5S with single-stranded DNA from adeno-associated virus. When the DNA was analyzed on neutral sucrose gradients, a single species was again observed which sedimented at 21S and was clearly distinct from 16S duplex adeno-associated virus DNA. A similar result was obtained even after incubation under annealing conditions, implying that the bulk of Aleutian disease virus virions contained a single non-complementary strand with a MW of about 1.4 .times. 106. Two major virus-associated polypeptides with MW of 89,100 and 77,600 were demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virus purified from infected cultures labeled with [35S]methionine. Aleutian disease virus is apparently a nondefective parvovirus.