Differences in cytokine gene expression profile between acute and secondary injury in adult rat spinal cord
Differences in cytokine gene expression profile between acute and secondary injury in adult rat spinal cord
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DOI：
10.1016/s0014-4886(03)00361-3
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发表时间：
2003-11-01
影响因子：
5.3
通讯作者：
Bregman, BS
中科院分区：
文献类型：
--
作者：
Nakamura, M;Houghtling, RA;Bregman, BS
损伤脊髓内的环境可能影响胚胎脊髓移植物支持轴突生长的能力。我们最近已经证明，胎儿脊髓移植和神经营养因子施用支持脊髓横断后的轴突再生，并且当这些治疗在损伤后延迟数周时，轴突生长的距离和量更大（Coumans等人，2001年）。在这项研究中，我们试图确定急性损伤的脊髓和二次损伤和再切除后的脊髓之间是否存在炎症介质的差异，这可以为轴突的再生长提供更有利的环境。本研究的结果表明，更快速的诱导转化生长因子（TGF）β 1 mRNA的表达在再损伤的脊髓比急性损伤的脊髓和衰减的促炎细胞因子mRNA的表达。此外，有一个快速的招聘，在变性白色物质吻和尾部的损伤，但较少的损伤部位本身的激活小胶质细胞/巨噬细胞。这些发现表明，与急性损伤的脊髓相比，再损伤的脊髓中TGF-1基因表达的增强和促炎细胞因子基因表达的减弱以及活化的小胶质细胞/巨噬细胞的分布改变可能为移植和轴突再生创造更有利的环境。(C)2003年爱思唯尔公司All rights reserved.
It is likely that the environment within the injured spinal cord influences the capacity of fetal spinal cord transplants to support axonal growth. We have recently demonstrated that fetal spinal cord transplants and neurotrophin administration support axonal regeneration after spinal cord transection, and that the distance and amount of axonal growth is greater when these treatments are delayed by several weeks after injury (Coumans et al., 2001). In this study, we sought to determine whether differences in inflammatory mediators exist between the acutely injured spinal cord and the spinal cord after a second injury and re-section, which could provide a more favorable environment for the axonal re-growth. The results of this study show a more rapid induction of transforming growth factor (TGF) beta1 mRNA expression in the re-injured spinal cord than the acutely injured spinal cord and an attenuation of proinflammatory cytokine mRNA expression. Furthermore, there was a rapid recruitment of activated microglia/macrophages in the degenerating white matter rostral and caudal to the injury but fewer within the lesion site itself. These findings suggest that the augmentation of TGFP-1 gene expression and the attenuation of pro-inflammatory cytokine gene expression combined with an altered distribution of activated microglia/macrophages in the re-injured spinal cord might create a more favorable milieu for transplants and axonal regrowth as compared to the acutely injured spinal cord. (C) 2003 Elsevier Inc. All rights reserved.