Association of Wnt2 and sFRP4 Expression in the Third Trimester Placenta in Women With Severe Preeclampsia
Association of Wnt2 and sFRP4 Expression in the Third Trimester Placenta in Women With Severe Preeclampsia
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DOI：
10.1177/1933719112472740
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发表时间：
2013-08-01
影响因子：
2.9
通讯作者：
Gao, Yan
中科院分区：
文献类型：
--
作者：
Zhang, Zhan;Zhang, Lin;Gao, Yan
背景：Wnt信号通路是一条保守通路，在调节滋养层功能中发挥着至关重要的作用。 Wnt 通路的异常表达可能导致滋养层功能障碍，从而导致先兆子痫 (PE) 的发病机制。然而，关于Wnt通路与人类妊娠PE之间关系的公开数据很少。 目的：本研究的目的是探讨妊娠晚期胎盘中Wnt2和分泌型卷曲相关蛋白4（sFRP4）的表达模式，并评估胎盘Wnt2和sFRP4表达变化与严重PE之间的关系。结果：与对照组相比，PE胎盘中Wnt2信使RNA的相对表达显着下调，而sFRP4在两组间无显着差异。 IHC结果显示，Wnt2和sFRP4主要表达于绒毛合体滋养层和绒毛外滋养层，而对照组Wnt2的染色强度高于PE组，PE组的sFRP4染色强度高于对照组。 Western blotting结果与IHC结果一致。结论：在人妊娠晚期胎盘中检测到Wnt信号通路，胎盘Wnt2表达减少和sFRP4表达增加可能与严重PE的发病有关。
Background:The Wnt signaling pathway is a conserved pathway and plays a crucial role in regulating trophoblast functions. Abnormal expression of the Wnt pathway may result in the dysfunction of the trophoblast that can contribute to the pathogenesis of preeclampsia (PE). However, published data regarding the association between Wnt pathway and PE in human pregnancy is rare.Objective:The aims of this study were to investigate the expression pattern of Wnt2 and secreted frizzled-related protein 4 (sFRP4) in the third trimester human placenta and to evaluate the relationship between changes in placental Wnt2 and sFRP4 expression and severe PE.Methods:The expression of Wnt2 and sFRP4 in normal and severe PE placentas was examined using immunohistochemistry (IHC), real-time polymerase chain reaction, and Western blot.Results:Compared to the controls, the relative expression of Wnt2 messenger RNA was remarkably downregulated in the PE placentas, while there was no significant difference in sFRP4 between the 2 groups. The IHC indicated that Wnt2 and sFRP4 were expressed predominantly in the villous syncytiotrophoblast and the extravillous trophoblast, whereas Wnt2 in the control group showed higher staining intensity than in the PE group, and sFRP4 in the PE group had a higher staining intensity than in the control group. Furthermore, the results of the Western blots were consistent with the IHC.Conclusions:The Wnt signaling pathway was detected in human third trimester placentas, and the decreased placental expression of Wnt2 and increased placental expression of sFRP4 may be associated with the pathogenesis of severe PE.