A phosphinate-based red fluorescent probe for imaging the superoxide radical anion generated by RAW264.7 macrophages
A phosphinate-based red fluorescent probe for imaging the superoxide radical anion generated by RAW264.7 macrophages
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DOI：
10.1002/cbic.200600392
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发表时间：
2007-03-05
期刊：
影响因子：
3.2
通讯作者：
Tang, Bo
中科院分区：
文献类型：
--
作者：
Xu, Kehua;Liu, Xia;Tang, Bo
设计并合成了一种高选择性、高灵敏度、可透过细胞的红色荧光探针4 '，9'-双（二苯基膦基）萘荧光素（PNF-1），用于检测生物体系中的O-2（中心点-）。该探针的设计策略是基于O-2（中心点-）的亲核机制介导探针脱保护为萘并荧光素，其发射光谱正好处于生物体系中低背景荧光干扰的光谱区域。在用O-2（中心点-）处理时，探针表现出对O-2（中心点-）的强荧光响应和高选择性，而不是对其他活性氧物质或生物化合物。PNF-1的线性校准曲线显示检测限为0.1 nm O-2（中心点-）。这种新型的荧光探针允许通过共聚焦显微镜检测活细胞中O-2（中心点-）浓度的纳摩尔变化。
4',9'-Bis(diphenylphosphinyl)naphthofluorescein (PNF-1) has been designed and synthesized as a highly selective, sensitive, cell-permeable, red fluorescent probe for detecting O-2(center dot-) in biological systems. The design strategy for the probe is based on the nucleophilic mechanism of O-2(center dot-) to mediate deprotection of the probe to naphthofluorescein, the emission spectrum of which is just in the spectral region of low background fluorescence interference in biological systems. Upon treatment with O-2(center dot-), the probe exhibits a strong fluorescence response and high selectivity for O-2(center dot-), rather than other reactive oxygen species or biological compounds. A linear calibration curve for PNF-1 showed a detection limit of 0.1 nm O-2(center dot-). This new type of fluorescent probe allows nanomolar changes in O-2(center dot-) concentrations in living cells to be detected by confocal microscopy.