Isolation and preliminary characterization of a progestogen specific binding macromolecule from the 273,000 g supernatant of rat and rabbit uteri.
Isolation and preliminary characterization of a progestogen specific binding macromolecule from the 273,000 g supernatant of rat and rabbit uteri.
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从 273,000 g 大鼠和兔子宫上清液中分离并初步表征孕激素特异性结合大分子。
DOI：
10.1210/endo-90-2-496
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发表时间：
1972
期刊：
影响因子：
4.8
通讯作者：
C. Bariso
中科院分区：
文献类型：
--
作者：
J. McGuire;C. Bariso
已在大鼠和兔子宫的细胞质部分中鉴定出两种不同的孕酮结合大分子。其中之一显然是米尔格罗姆和鲍利厄早些时候报道的活页夹。它的结合并不具体。第二种大分子显然是由 McGuire 和 DeDella 以及 Milgrom 等人报道的。它显然只与孕激素结合，被称为孕激素受体。这两种结合剂可以根据结合特异性并通过硫酸铵分级分离。动力学研究表明存在两种结合剂。表征孕激素特异性结合剂的尝试表明，该结合剂是分子量约为85,000的蛋白质。组织特异性研究表明，这种结合剂存在于子宫和阴道的细胞质部分中，但不存在于脾、胃、肝、肾、肠、腿部肌肉或手臂肌肉的细胞质部分中。没有发现其存在于血液中的证据。最佳黄体酮结合发生在...
Two different progesterone binding macromolecules have been identified in the cytosol fraction of rat and rabbit uteri. One is apparently the binder earlier reported by Milgrom and Baulieu. It is not specific in its binding. The second macromolecule is apparently that which has been reported by McGuire and DeDella and Milgrom et al. It apparently binds only to progestogens and has been termed a progestogen receptor. These two binders can be separated on the basis of binding specificity and by ammonium sulfate fractionation. Kinetic studies indicate the presence of two binders. Attempts to characterize the progestogen specific binder demonstrate that the binder is a protein with a molecular weight of about 85,000. Tissue specificity studies indicate that this binder exists in the cytosol fraction of uterus and vagina but not in the cytosol fraction of spleen, stomach, liver, kidney, intestine, leg muscle, or arm muscle. No evidence was found for its presence in blood. Optimal progesterone binding occurs be...