The distribution of cartilage oligomeric matrix protein (COMP) in equine carpal articular cartilage and its variation with exercise and cartilage deterioration.
The distribution of cartilage oligomeric matrix protein (COMP) in equine carpal articular cartilage and its variation with exercise and cartilage deterioration.
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软骨寡聚基质蛋白（COMP）在马腕关节软骨中的分布及其随运动和软骨退化的变化。
DOI：
10.1053/tvjl.2001.0590
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发表时间：
2001
影响因子：
2.2
通讯作者：
A. Goodship
中科院分区：
文献类型：
--
作者：
R. Murray;Roger K. Smith;F. Henson;A. Goodship
根据以前的研究，肌腱接受最大的负荷已被证明具有最高水平的软骨寡聚基质蛋白（COMP），我们假设COMP在关节软骨中的分布可能会受到机械负荷的影响。本研究旨在（a）描述2岁纯种马腕关节软骨中部COMP免疫反应的模式;（B）确定地形变化;（c）比较高（组1）和低（组2）强度训练;（d）描述早期骨关节炎部位的COMP免疫反应。第1组（n =6）进行了19周的高强度跑步机训练计划，第2组（n =6）每天步行，直到安乐死。准备桡侧和第三腕关节面的背侧和掌侧部位。采用生物素-链霉亲和素/过氧化物酶法，用多克隆兔抗马COMP抗血清进行免疫组织化学。结果显示：（B）未观察到部位之间分布的差异，但在运动马（n =2）中总COMP水平确实在部位之间变化，在桡侧、中间和第三腕外侧小面上，背侧部位比掌侧部位含有更少的COMP;（c）与第2组相比，第1组软骨在深层显示出显著的区域间分布，其中染色在整个基质中更普遍，和（d）原纤维化软骨显示出基质中局部免疫反应性增加。这些结果表明，马COMP分布的地带性变化可能受到负荷的影响。
Based on previous studies where tendons receiving the most load have been shown to have the highest levels of cartilage oligomeric matrix protein (COMP), we hypothesized that COMP distribution in articular cartilage may be influenced by mechanical loading. This investigation aimed (a) to describe the pattern of COMP immunoreactivity in middle carpal joint cartilage of two-year-old Thoroughbred horses; (b) to determine topographical variations; (c) to compare high (group 1) and low (group 2) intensity training and (d) to describe COMP immunoreactivity at sites with early osteoarthritis. Group 1 (n =6) underwent a 19 week high-intensity treadmill training programme and group 2 (n =6) were given daily walking until euthanasia. Dorsal and palmar sites on radial and third carpal articular surfaces were prepared. Immunohistochemistry was performed with polyclonal rabbit anti-equine COMP antiserum using a biotin-streptavidin/peroxidase method. Results showed: (a) intracellular immunoreactivity was present in all cartilage zones, but the distribution of COMP staining within the matrix varied between cartilage zones; (b) differences in distribution between sites were not observed, but total COMP levels in exercised horses (n =2) did vary between sites with dorsal sites containing less COMP than palmar sites on the radial, intermediate and third carpal lateral facet; (c) group 1 cartilage showed marked interterritorial distribution in the deep layer compared to group 2 where staining was more generalized throughout the matrix and (d) fibrillated cartilage showed increased local immunoreactivity in the matrix. These findings demonstrate zonal variations in equine COMP distribution which may be influenced by loading.
正常关节软骨浅层和深层的纤连蛋白合成。
影响因子：
--
作者：
Hayashi,T;Abe,E;Jasin,HE
通讯作者：
Jasin,HE