Killing of Schistosoma mansoni sporocysts by Biomphalaria glabrata hemolymph in vitro: Alteration of hemocyte behavior after poly-L-lysine treatment of plastic, and the kinetics of killing by different host strains
Killing of Schistosoma mansoni sporocysts by Biomphalaria glabrata hemolymph in vitro: Alteration of hemocyte behavior after poly-L-lysine treatment of plastic, and the kinetics of killing by different host strains
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DOI：
10.2307/3284173
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发表时间：
1996-04-01
影响因子：
1.3
通讯作者：
Bayne, CJ
中科院分区：
文献类型：
--
作者：
Boehmler, AM;Fryer, SE;Bayne, CJ
与未处理的塑料相比，0.1 mg/ml聚l -赖氨酸处理的底物显著改变了腹足软体动物Biomphalaria glabrata的血细胞在塑料上的行为。在赖氨酸上，细胞扩散最小，移动明显加快，并形成聚集体。采用细胞介导的细胞毒性（CMC）实验，比较了修饰后和未处理的底物对曼氏血吸虫孢子囊的杀伤能力。1316-R1（抗性）钉螺血淋巴在赖氨酸处理的井中有较高的杀伤效果；在处理和未处理的井中，MO（易感）血淋巴的孢子囊死亡率无显著差异。采用聚l -赖氨酸处理塑料的CMC测定方法，比较了2个敏感寄主菌株（MO、MRLc）和2个耐药寄主菌株（l316-R1、10R2）在血淋巴中的杀虫动力学。在两种耐药的蜗牛菌株之间可以观察到明显的差异，这表明寄生虫识别、杀死或两者兼而有之的机制不同。
Behavior of hemocytes of the gastropod molluse Biomphalaria glabrata was markedly changed on plastic by treatment of the substrate with 0.1 mg/ml poly-L-lysine compared to behavior on untreated plastic. On lysine, the cells showed minimal spreading, moved significantly faster, and formed aggregates. Cell-mediated cytotoxicity (CMC) assays were set up on the modified and untreated substrates to compare the killing capacities of B. glabrata hemocytes against Schistosoma mansoni sporocysts. Hemolymph from 1316-R1 (resistant) snails showed higher killing in lysine-treated wells; no significant difference in sporocyst mortality was observed in MO (susceptible) hemolymph between treated and untreated wells. The CMC assays on poly-L-lysine-treated plastic were used to compare the kinetics of parasite killing in hemolymph from 2 susceptible (MO, MRLc) and 2 resistant(l316-R1, 10R2) host strains. Marked differences could be observed between the two resistant snail strains, suggesting different mechanisms of parasite recognition, killing, or both.