Acceleration of Skin Wound-Healing Reactions by Autologous Micrograft Tissue Suspension
Acceleration of Skin Wound-Healing Reactions by Autologous Micrograft Tissue Suspension
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DOI：
10.3390/medicina56070321
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发表时间：
2020-07-01
影响因子：
2.6
通讯作者：
Saparov, Arman
中科院分区：
文献类型：
--
作者：
Jimi, Shiro;Takagi, Satoshi;Saparov, Arman
背景和目的：皮肤移植是重建外科中常用的促进皮肤再生的方法。这种方法经常导致意外的疤痕形成。我们以前表明，皮肤伤口愈合在正常小鼠是由微移植（MG）技术加速。目前，已经利用这种技术进行了临床试验;然而，这种方法的有益效果背后的驱动机制仍不清楚。在本研究中，我们专注于五个主要的组织反应，在伤口愈合，即再生，迁移，肉芽，新生血管和contractions.Methods：形态计量学分析进行组织样本从背部伤口的小鼠。结果：创面面积与肉芽组织的大小和新生血管密度相关。肉芽组织中的血管分布分析表明，MG组的新生血管延伸并到达表皮下区域，而对照组仅发育一半。此外，MG增强了上皮再生和迁移。肌成纤维细胞是使用α-平滑肌肌动蛋白丝的用于伤口收缩的已知机制。它们在肉芽组织中的分布主要发现再生上皮下方，并在MG group.Conclusions显着进展：这些研究结果表明，MG加速了一系列的伤口愈合反应，并可用于治疗难治性伤口的临床情况。
Background and objectives: Skin grafting is a method usually used in reconstructive surgery to accelerate skin regeneration. This method results frequently in unexpected scar formations. We previously showed that cutaneous wound-healing in normal mice is accelerated by a micrograft (MG) technique. Presently, clinical trials have been performed utilizing this technology; however, the driving mechanisms behind the beneficial effects of this approach remain unclear. In the present study, we focused on five major tissue reactions in wound-healing, namely, regeneration, migration, granulation, neovascularization and contraction.Methods:Morphometrical analysis was performed using tissue samples from the dorsal wounds of mice. Granulation tissue formation, neovascularization and epithelial healing were examined.Results:The wound area correlated well with granulation sizes and neovascularization densities in the granulation tissue. Vascular distribution analysis in the granulation tissue indicated that neovessels extended and reached the subepidermal area in the MG group but was only halfway developed in the control group. Moreover, epithelialization with regeneration and migration was augmented by MG. Myofibroblast is a known machinery for wound contraction that uses alpha-smooth muscle actin filaments. Their distribution in the granulation tissue was primarily found beneath the regenerated epithelium and was significantly progressed in the MG group.Conclusions:These findings indicated that MG accelerated a series of wound-healing reactions and could be useful for treating intractable wounds in clinical situations.