Search for protein partners of mitochondrial single-stranded DNA-binding protein Rim1p using a yeast two-hybrid system.
Search for protein partners of mitochondrial single-stranded DNA-binding protein Rim1p using a yeast two-hybrid system.
复制标题
使用酵母双杂交系统寻找线粒体单链 DNA 结合蛋白 Rim1p 的蛋白伴侣。
DOI：
10.1007/bf02930953
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发表时间：
2003
影响因子：
2.6
通讯作者：
Foury,F
中科院分区：
文献类型：
--
作者：
Kucejová,B;Foury,F
rim1是酵母（酿酒酵母）的一个核基因，编码一种具有单链dna结合活性的蛋白质，这种蛋白质对线粒体基因组的维持至关重要。到目前为止，在酵母中还没有发现Rim1p的蛋白伴侣。为了更好地了解该蛋白在线粒体DNA复制和重组中的作用，通过酵母双杂交系统寻找蛋白质相互作用物。这种方法鉴定出了几种候选基因，包括一种假定的转录因子Azf1p和一种具有RNA解旋酶结构域的蛋白质Mph1p，这种蛋白质已知会影响细胞核和线粒体基因组的突变率。
RIM1is a nuclear gene of the yeastSaccharomyces cerevisiaecoding for a protein with single-stranded DNA-binding activity that is essential for mitochondrial genome maintenance. No protein partners of Rim1p have been described so far in yeast. To better understand the role of this protein in mitochondrial DNA replication and recombination, a search for protein interactors by the yeast two-hybrid system was performed. This approach led to the identification of several candidates, including a putative transcription factor, Azf1p, and Mph1p, a protein with an RNA helicase domain which is known to influence the mutation rate of nuclear and mitochondrial genomes.