Immunohistological analysis of HPV L1 capsid protein and p16 protein in low-grade dysplastic lesions of the uterine cervix
Immunohistological analysis of HPV L1 capsid protein and p16 protein in low-grade dysplastic lesions of the uterine cervix
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DOI：
10.1016/j.prp.2010.09.005
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Fukuda, Toshio
中科院分区：
文献类型：
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作者：
Hoshikawa, Satomi;Sano, Takaaki;Fukuda, Toshio
人乳头瘤病毒（HPV）L1衣壳蛋白（L1）与HPV感染的生产期有关。然而，L1的表达及其与p16表达（HPV感染的替代标志物）的关系尚不清楚。我们研究了宫颈上皮内瘤变中L1和p16表达之间的关系。将组织分为四类：退行性病变（n = 48）、进行性病变（n = 40）和随机选择的CIN 1 -2（n = 67）和CIN 3（n = 44）。P16在进展组中的阳性率显著高于消退组，在CIN 3组中的阳性率显著高于其他各组。L1阳性在各类别之间不显著。将其分为L1-/p16-、L1+/p16-、L1 +/p16+、L1-/p16+四组。L1-/p16-模式与CIN 1 -2的消退性质显著相关。可能存在一些显示L1+/p16+特征的CIN 3病例，这些病例仍然是HPV产生的。联合检测L1和p16可能有助于评估低度宫颈不典型增生的进展风险。(c)2010年Elsevier GmbH。All rights reserved.
Human papillomavirus (HPV) L1 capsid protein (L1) is associated with the productive phase of HPV infection. However, the expression of L1 and its relationship to p16 expression, a surrogate marker for HPV infection, are unknown. We examined the relationship between L1 and p16 expression in cervical intraepithelial neoplasia. Tissues were divided into four categories: regressive lesions (n = 48), progressive lesions (n = 40), and randomly selected CIN1-2 (n = 67) and CIN3 (n = 44). P16 positivity in the progression cases was significantly higher than that in the regression cases, and p16 positivity in the CIN3 cases was significantly higher than that in any other categories. L1 positivity was not significant between each category. The staining pattern was divided into the following four groups: L1-/p16-, L1+/p16-, L1+/p16+, L1-/p16+. The L1-/p16- pattern was significantly associated with the regression nature in CIN1-2. Some CIN3 cases showing a feature of L1+/p16+, which are still HPV-productive, are likely to exist. The combination of both L1 and p16 may be useful in the evaluation of the progression risk of low-grade cervical dysplasia. (c) 2010 Elsevier GmbH. All rights reserved.