PADLOCK PROBES - CIRCULARIZING OLIGONUCLEOTIDES FOR LOCALIZED DNA DETECTION
PADLOCK PROBES - CIRCULARIZING OLIGONUCLEOTIDES FOR LOCALIZED DNA DETECTION
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DOI：
10.1126/science.7522346
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发表时间：
1994-09-30
期刊：
影响因子：
56.9
通讯作者：
LANDEGREN, U
中科院分区：
文献类型：
--
作者：
NILSSON, M;MALMGREN, H;LANDEGREN, U
来自人类和其他基因组DNA片段的核苷酸序列信息正在迅速积累。然而，使用如此短的DNA片段来鉴定基因组文库中的克隆或全基因组印迹中的片段或染色体的原位分析经常被证明是困难的。设计了由两个目标互补片段组成的寡核苷酸探针，并通过一个连接子序列连接。在识别特定的核酸分子后，探针的末端通过连接酶的作用连接起来，形成与目标序列相连的环状DNA分子。因此，这些探针在最小的背景下提供高度特异性的检测。
Nucleotide sequence information derived from DNA segments of the human and other genomes is accumulating rapidly. However, it frequently proves difficult to use such short DNA segments to identify clones in genomic libraries or fragments in blots of the whole genome or for in situ analysis of chromosomes. Oligonucleotide probes, consisting of two target-complementary segments, connected by a linker sequence, were designed. Upon recognition of the specific nucleic acid molecule the ends of the probes were joined through the action of a ligase, creating circular DNA molecules catenated to the target sequence. These probes thus provide highly specific detection with minimal background.