Antimicrobial Activity of Curcumin against Helicobacter pylori Isolates from India and during Infections in Mice
Antimicrobial Activity of Curcumin against Helicobacter pylori Isolates from India and during Infections in Mice
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DOI：
10.1128/aac.01242-08
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发表时间：
2009-04-01
影响因子：
4.9
通讯作者：
Mukhopadhyay, Asish K.
中科院分区：
文献类型：
--
作者：
De, Ronita;Kundu, Parag;Mukhopadhyay, Asish K.
治疗失败是幽门螺杆菌相关性胃十二指肠疾病如胃炎、消化性溃疡和胃癌的主要原因。姜黄素是从姜黄中提取的二阿魏酰甲烷，最近被证明可以抑制H。幽门生长姜黄素对65株临床分离的H. pylori的体外培养和抗H. pylori感染进行了检查。姜黄素的MIC范围为5 μ g/ml至50 μ g/ml，显示其抑制H.幽门螺杆菌的体外生长，而不管菌株的遗传组成。aroE基因的核苷酸序列，编码莽草酸脱氢酶，姜黄素似乎作为一个非竞争性抑制剂，从H。pylori菌株呈现不同的姜黄素MIC，表明姜黄素介导的印度H.幽门螺杆菌菌株可能并不总是依赖于莽草酸途径。姜黄素对H.幽门螺杆菌感染的C57 BL/6小鼠的胃粘膜损伤及其在减少由于感染引起的胃损伤方面的功效进行了组织学检查。姜黄素对H. pylori感染，对根除H. pylori感染的小鼠以及H.幽门引起的胃损伤本研究为姜黄素抗H. pylori感染，表明其作为替代疗法的潜力，并为进一步研究姜黄素的新型抗菌靶标的鉴定开辟了道路。
Treatment failure is a major cause of concern for the Helicobacter pylori-related gastroduodenal diseases like gastritis, peptic ulcer, and gastric cancer. Curcumin, diferuloylmethane from turmeric, has recently been shown to arrest H. pylori growth. The antibacterial activity of curcumin against 65 clinical isolates of H. pylori in vitro and during protection against H. pylori infection in vivo was examined. The MIC of curcumin ranges from 5 mu g/ml to 50 mu g/ml, showing its effectiveness in inhibiting H. pylori growth in vitro irrespective of the genetic makeup of the strains. The nucleotide sequences of the aroE genes, encoding shikimate dehydrogenase, against which curcumin seems to act as a noncompetitive inhibitor, from H. pylori strains presenting differential curcumin MICs showed that curcumin-mediated growth inhibition of Indian H. pylori strains may not be always dependent on the shikimate pathway. The antimicrobial effect of curcumin in H. pylori-infected C57BL/6 mice and its efficacy in reducing the gastric damage due to infection were examined histologically. Curcumin showed immense therapeutic potential against H. pylori infection as it was highly effective in eradication of H. pylori from infected mice as well as in restoration of H. pylori-induced gastric damage. This study provides novel insights into the therapeutic effect of curcumin against H. pylori infection, suggesting its potential as an alternative therapy, and opens the way for further studies on identification of novel antimicrobial targets of curcumin.