Involvement of ethylene signaling in zinc oxide nanoparticle-mediated biochemical changes in Arabidopsis thaliana leaves
Involvement of ethylene signaling in zinc oxide nanoparticle-mediated biochemical changes in Arabidopsis thaliana leaves
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DOI：
10.1039/c8en00971f
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发表时间：
2019-01-01
影响因子：
7.3
通讯作者：
Gan, Yinbo
中科院分区：
文献类型：
--
作者：
Khan, Ali Raza;Wakeel, Abdul;Gan, Yinbo
金属纳米颗粒在工业中的应用日益广泛，导致其在农业用地上的积累，对全球农作物的产量和质量构成严重威胁。各种报道表明植物通过激素来应对环境胁迫，然而，ZnO NP胁迫下乙烯的调节仍然是未知的。在这项研究中，乙烯信号缺陷型突变体（etr 1 -3和ein 2 -1）进行了研究比较，拟南芥野生型下ZnO纳米粒子的压力。我们发现ZnO纳米颗粒通过调控细胞周期相关基因的表达，显著抑制拟南芥的生长并诱导其毒性。更重要的是，我们的研究结果表明，乙烯信号和生物合成在这一过程中的参与。ZnO纳米颗粒影响乙烯不敏感突变体和野生型植物的生物量、叶绿素和糖含量。较高的ROS积累和增加的脂质过氧化反应表明ZnO纳米颗粒诱导拟南芥的毒性。观察到的高抗氧化酶活性和mRNA转录水平，其相应的基因在突变体植物中清楚地表明，乙烯不敏感突变体的耐受性相比，野生型植物对氧化损伤所造成的ZnO NP应力。总的来说，我们的研究结果表明，乙烯不敏感的突变体耐受ZnO NP诱导的应力比WT更有效。这些结果表明，乙烯诱导氧化损伤植物在ZnO NP胁迫下。
The growing use of metallic nanoparticles in industry has resulted in their accumulation in agricultural land, which poses a serious threat to the yield and quality of crops worldwide. Various reports showed that plants face environmental stress through hormones; however, the regulation of ethylene under ZnO NP stress is still unknown. In this study, ethylene-signaling defective mutants (etr1-3 and ein2-1) were studied in comparison to Arabidopsis wild-type under ZnO NPs stress. We found that ZnO NPs significantly inhibited the growth of Arabidopsis and induced toxicity by regulating the expression of cell cycle-related genes. More importantly our results showed the involvement of ethylene signaling and biosynthesis in this process. The ZnO NPs affected the biomass, and chlorophyll and sugar contents in both the ethylene-insensitive mutants and wild-type plants. The higher ROS accumulation and increased lipid peroxidation showed that the ZnO NPs induced toxicity in Arabidopsis. The observed high antioxidant enzyme activities and mRNA transcript levels of their corresponding genes in the mutant plants clearly showed the tolerance of the ethylene-insensitive mutants compared to the wild-type plant against oxidative damage caused by ZnO NP stress. Overall, our results showed that the ethylene-insensitive mutants tolerated ZnO NP-induced stress more efficiently than WT. These results suggest that ethylene induces oxidative damage in plants under ZnO NP stress.