A.actinomycetemcomitans-induced periodontal disease promotes systemic and local responses in rat periodontium.
A.actinomycetemcomitans-induced periodontal disease promotes systemic and local responses in rat periodontium.
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DOI：
10.1111/j.1600-051x.2011.01847.x
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发表时间：
2012-04
影响因子：
6.7
通讯作者：
Graves DT
中科院分区：
文献类型：
--
作者：
Bezerra Bde B;Andriankaja O;Kang J;Pacios S;Bae HJ;Li Y;Tsiagbe V;Schreiner H;Fine DH;Graves DT
目的探讨放线菌绦虫（Aa）感染后的组织学和细胞反应。在基线和感染后4,5或6周，评估感染Aa的Wistar大鼠的抗体反应、口服Aa定植、附着丧失、PMN募集、连接上皮和结缔组织中的TNF-α、破骨细胞和局部淋巴结的适应性免疫反应。部分组在第4周给予抗菌治疗。感染4周内出现Aa抗体应答，78%接种大鼠口腔可检出Aa （p<0.05）。Aa感染显著增加附着丧失（p<0.05）。TNF-α在结膜上皮中的表达规律相同。Aa刺激结缔组织中破骨细胞的形成和TNF-α的高表达（p<0.05）。Aa感染后PMN募集明显增加（p<0.05）。Aa能增加CD8+ T细胞的数量（p<0.05），但不能增加CD4+ T细胞和调节性T细胞（Tregs）的数量（p<0.05）。Aa感染刺激了局部反应，增加了连接上皮中pmn和TNF-α的表达，并失去了附着。TNF-a在乙脑中的表达和附着丧失均可通过抗生素治疗逆转。Aa感染使结缔组织中TNF-α、破骨细胞数量、淋巴结中CD8+ T细胞增加。结果将Aa感染与牙周破坏的重要特征联系起来。
To characterize the histologic and cellular response to A. actinomycetemcomitans (Aa) infection. Wistar rats infected with Aa were evaluated for antibody response, oral Aa colonization, loss of attachment, PMN recruitment, TNF-α in the junctional epithelium and connective tissue, osteoclasts, and adaptive immune response in local lymph nodes at baseline and 4, 5 or 6 weeks after infection. Some groups were given antibacterial treatment at 4 weeks. An antibody response against Aa occurred within 4 weeks of infection and 78% of inoculated rats had detectable Aa in the oral cavity (p<0.05). Aa infection significantly increased loss of attachment which was reversed by antibacterial treatment (p<0.05). TNF-α expression in the junctional epithelium followed the same pattern. Aa stimulated high osteoclast formation and TNF-α expression in the connective tissue (p<0.05). PMN recruitment significantly increased after Aa infection (p<0.05). Aa also increased the number of CD8+ T cells (p<0.05) but not CD4+ T cells or regulatory T cells (Tregs) (p>0.05). Aa infection stimulated a local response which increased numbers of PMNs and TNF-α expression in the junctional epithelium and loss of attachment. Both TNF-a expression in JE and loss of attachment was reversed by antibiotic treatment. Aa infection also increased TNF-α in the connective tissue, osteoclast numbers, CD8+ T cells in lymph nodes. The results link Aa infection with important characteristics of periodontal destruction.