INHIBITION OF PROTEIN-SERINE THREONINE PHOSPHATASES DIRECTLY INDUCES PREMATURE CHROMOSOME CONDENSATION IN MAMMALIAN SOMATIC-CELLS
INHIBITION OF PROTEIN-SERINE THREONINE PHOSPHATASES DIRECTLY INDUCES PREMATURE CHROMOSOME CONDENSATION IN MAMMALIAN SOMATIC-CELLS
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DOI：
10.2220/biomedres.16.63
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发表时间：
1995-02-01
影响因子：
1.2
通讯作者：
KOSAKA, H
中科院分区：
文献类型：
--
作者：
GOTOH, E;ASAKAWA, Y;KOSAKA, H
染色体在有丝分裂过程中常发生凝聚，但在某些情况下凝聚可从有丝分裂过程中解偶联。这种现象被称为过早染色体凝聚（PCC）。在这里，我们表明，100 nM冈田酸或calyculin A可以在几种类型的哺乳动物细胞的细胞周期中的任何时间诱导PCC;观察到G1-，S-和G2-PCC。冈田酸和calyculin A都是1型和2A型蛋白丝氨酸/苏氨酸磷酸酶的特异性抑制剂，表明这些磷酸酶在调节染色体凝聚中起着核心作用。
Chromosomes usually condense during mitosis, but condensation may be uncoupled from mitotic events under certain circumstances. This phenomenon is known as premature chromosome condensation (PCC). Here we show that 100 nM okadaic acid or calyculin A can induce PCC at any time during the cell cycle of several types of mammalian cells; G1-, S- and G2-PCC were observed. Both okadaic acid and calyculin A are specific inhibitors of type 1 and type 2A protein serine/threonine phosphatases, suggesting that these phosphatases play a central role in regulating chromosome condensation.