Characterization of the Ileal Microbiota in Rejecting and Nonrejecting Recipients of Small Bowel Transplants
Characterization of the Ileal Microbiota in Rejecting and Nonrejecting Recipients of Small Bowel Transplants
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DOI：
10.1111/j.1600-6143.2011.03860.x
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发表时间：
2012-03-01
影响因子：
8.8
通讯作者：
Mercer, D. F.
中科院分区：
文献类型：
--
作者：
Oh, P. L.;Martinez, I.;Mercer, D. F.
对于患有不可逆性肠衰竭的患者，小肠移植可以成为一种挽救生命的方法。同种异体移植排斥反应仍然是发病率和死亡率的主要来源，其准确的诊断和治疗至关重要。在这项研究中，我们使用16S核糖体RNA基因标签的焦磷酸测序来比较小肠移植患者在非排斥、预排斥和活动性排斥状态下回肠微生物区系的组成。在排斥发作期间，细菌门(p<；0.001)和乳杆菌目(p<；0.005)的比例显著减少，变形杆菌门，尤其是肠杆菌科的比例显著增加(p<；0.005)。受试者工作特性分析表明，回肠流出物中几种细菌分类群的相对比例，特别是Firmicuts，可以用来区分非排斥和主动排斥。总之，本研究的结果表明，小肠移植排斥反应与回肠流出物中微生物种群的变化有关，并支持微生物群分析作为潜在的排斥诊断生物标志物。未来的研究应该调查我们观察到的生物失调是排斥过程的原因还是结果。
Small bowel transplantation can be a life-preserving procedure for patients with irreversible intestinal failure. Allograft rejection remains a major source of morbidity and mortality and its accurate diagnosis and treatment are critical. In this study, we used pyrosequencing of 16S ribosomal RNA gene tags to compare the composition of the ileal microbiota present during nonrejection, prerejection and active rejection states in small bowel transplant patients. During episodes of rejection, the proportions of phylum Firmicutes (p < 0.001) and the order Lactobacillales (p < 0.01) were significantly decreased, while those of the phylum Proteobacteria, especially the family Enterobacteriaceae, were significantly increased (p < 0.005). Receiver-operating characteristic analysis revealed that relative proportions of several bacterial taxa in ileal effluents and especially Firmicutes, could be used to discriminate between nonrejection and active rejection. In conclusion, the findings obtained during this study suggest that small bowel transplant rejection is associated with changes in the microbial populations in ileal effluents and support microbiota profiling as a potential diagnostic biomarker of rejection. Future studies should investigate if the dysbiosis that we observed is a cause or a consequence of the rejection process.